PXD002234

PXD002234 is an original dataset announced via ProteomeXchange.

Title	Influenza Polymerase Can Adopt an Alternative Configuration Involving a Radical Repacking of PB2 Domains
Description	Influenza virus polymerase transcribes or replicates the segmented RNA genome (vRNA) into respectively viral mRNA or full-length copies and initiates RNA synthesis by binding the conserved 3' and 5' vRNA ends (the promoter). In recent structures of promoter-bound polymerase, the cap-binding and endonuclease domains are configured for cap snatching, which generates capped transcription primers. Here, we present a FluB polymerase structure with a bound complementary cRNA 5' end that exhibits a major rearrangement of the subdomains within the C-terminal two-thirds of PB2 (PB2-C). Notably, the PB2 nuclear localization signal (NLS)- containing domain translocates ~90 A ̊ to bind to the endonuclease domain. FluA PB2-C alone and RNA-free FluC polymerase are similarly arranged. Biophysical and cap-dependent endonuclease assays show that in solution the polymerase explores different conformational distributions depending on which RNA is bound. The inherent flexibility of the polymerase allows it to adopt alternative conformations that are likely important during polymerase maturation into active progeny RNPs. Extra contact information: Stephen Cusack, EMBL Grenoble Outstation, Unit of Virus Host-Cell Interactions, France ( corresponding author and lab head )
HostingRepository	PRIDE
AnnounceDate	2016-01-05
AnnouncementXML	Submission_2016-01-05_00:30:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Bock
SpeciesList	scientific name: Influenza A virus (A/Vietnam/1203/04(H5N1)); NCBI TaxID: 284218; scientific name: Influenza B virus (B/Memphis/13/03); NCBI TaxID: 1601067;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-26 23:52:43	ID requested
1	2016-01-04 12:21:38	announced
⏵ 2	2016-01-05 00:30:51	announced	Updated project metadata.

Thierry E, Guilligay D, Kosinski J, Bock T, Gaudon S, Round A, Pflug A, Hengrung N, El Omari K, Baudin F, Hart DJ, Beck M, Cusack S, Influenza Polymerase Can Adopt an Alternative Configuration Involving a Radical Repacking of PB2 Domains. Mol Cell, 61(1):125-37(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Influenza, polymerase, XL-MS

Martin Beck
contact affiliation	EMBL, SCB, Heidelberg, Germany
contact email	martin.beck@embl.de
lab head
Thomas Bock
contact affiliation	EMBL, SCB, Heidelberg, Germany
contact email	thomas.bock@embl.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002234
     1. Label: PRIDE project
     2. Name: Influenza Polymerase Can Adopt an Alternative Configuration Involving a Radical Repacking of PB2 Domains