PXD002227

PXD002227 is an original dataset announced via ProteomeXchange.

Title	An effective plasma membrane proteomics approach for small tissue samples
Description	Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of ? 1600 proteins of which > 60% are known to be associated to the plasma membrane according to GO annotations, including (G-protein coupled) receptors, ion channels and transporters, and this from mm3-scale tissue.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:33:09.306.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dirk Valkenborg
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-26 02:59:01	ID requested
1	2022-02-22 03:42:47	announced
⏵ 2	2024-10-22 05:33:11	announced	2024-10-22: Updated project metadata.

10.1038/srep10917;

Smolders K, Lombaert N, Valkenborg D, Baggerman G, Arckens L, An effective plasma membrane proteomics approach for small tissue samples. Sci Rep, 5():10917(2015) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Orbitrap Velos, affinity-purification, mice, Acute slice biotinylation assay, membrane proteomics,Shotgun proteomics

Dirk Valkenborg
contact affiliation	Center for Proteomics, University of Antwerp, Belgium Applied Bio & molecular Systems, VITO, Belgium.
contact email	dirk.valkenborg@vito.be
lab head
Dirk Valkenborg
contact affiliation	VITO
contact email	dirk.valkenborg@vito.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD002227

PRIDE project URI

• PRIDE
  1. PXD002227
     1. Label: PRIDE project
     2. Name: An effective plasma membrane proteomics approach for small tissue samples