PXD002227 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An effective plasma membrane proteomics approach for small tissue samples |
Description | Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of ? 1600 proteins of which > 60% are known to be associated to the plasma membrane according to GO annotations, including (G-protein coupled) receptors, ion channels and transporters, and this from mm3-scale tissue. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:33:09.306.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dirk Valkenborg |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | biotinylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-05-26 02:59:01 | ID requested | |
1 | 2022-02-22 03:42:47 | announced | |
⏵ 2 | 2024-10-22 05:33:11 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/srep10917; |
Smolders K, Lombaert N, Valkenborg D, Baggerman G, Arckens L, An effective plasma membrane proteomics approach for small tissue samples. Sci Rep, 5():10917(2015) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Orbitrap Velos, affinity-purification, mice, Acute slice biotinylation assay, membrane proteomics,Shotgun proteomics |
Contact List
Dirk Valkenborg |
contact affiliation | Center for Proteomics, University of Antwerp, Belgium Applied Bio & molecular Systems, VITO, Belgium. |
contact email | dirk.valkenborg@vito.be |
lab head | |
Dirk Valkenborg |
contact affiliation | VITO |
contact email | dirk.valkenborg@vito.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD002227 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002227
- Label: PRIDE project
- Name: An effective plasma membrane proteomics approach for small tissue samples