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PXD002227

PXD002227 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn effective plasma membrane proteomics approach for small tissue samples
DescriptionAdvancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of ? 1600 proteins of which > 60% are known to be associated to the plasma membrane according to GO annotations, including (G-protein coupled) receptors, ion channels and transporters, and this from mm3-scale tissue.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:33:09.306.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDirk Valkenborg
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListbiotinylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-05-26 02:59:01ID requested
12022-02-22 03:42:47announced
22024-10-22 05:33:11announced2024-10-22: Updated project metadata.
Publication List
10.1038/srep10917;
Smolders K, Lombaert N, Valkenborg D, Baggerman G, Arckens L, An effective plasma membrane proteomics approach for small tissue samples. Sci Rep, 5():10917(2015) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Orbitrap Velos, affinity-purification, mice, Acute slice biotinylation assay, membrane proteomics,Shotgun proteomics
Contact List
Dirk Valkenborg
contact affiliationCenter for Proteomics, University of Antwerp, Belgium Applied Bio & molecular Systems, VITO, Belgium.
contact emaildirk.valkenborg@vito.be
lab head
Dirk Valkenborg
contact affiliationVITO
contact emaildirk.valkenborg@vito.be
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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