PXD002226

PXD002226 is an original dataset announced via ProteomeXchange.

Title	Caenorhabditis elegans mRBPome
Description	This project aims to identify novel RNA binding proteins in the nematode, Caenorhabditis elegans. Since interactions between RNAs and proteins may be transient, these animals were crosslinked with UV light at 254 nm which promotes the covalent link between proteins and RNAs. After this, polyadenylated mRNAs were purified via oligo(dT) coupled to magentic beads under stringent conditions. Finally, samples were subjected to mass spectrometry analysis. To rule out the possibility of RNA-independent binding we also analysed other samples: i) samples digested with RNase one; ii) samples where we performed competition assays with polyadenylic acid
HostingRepository	PRIDE
AnnounceDate	2015-10-26
AnnouncementXML	Submission_2015-12-08_04:07:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Matia Gonzalez
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-26 02:56:59	ID requested
1	2015-10-26 05:33:30	announced
⏵ 2	2015-12-08 04:07:51	announced	Updated publication reference for PubMed record(s): 26595419.

Matia-Gonz, á, lez AM, Laing EE, Gerber AP, Conserved mRNA-binding proteomes in eukaryotic organisms. Nat Struct Mol Biol, 22(12):1027-33(2015) [pubmed]

curator keyword: Biological

submitter keyword: Caenorhabditis elegans, apoptosis, RBP, LC-MS/MS

André P. Gerber
contact affiliation	Dept. of Microbial and Cellular Sciences Faculty of Health and Medical Sciences University of Surrey Stag Hill Campus 19 AX 01 Guildford, GU2 7XH U.K.
contact email	a.gerber@surrey.ac.uk
lab head
Ana Matia Gonzalez
contact affiliation	University of Surrey
contact email	a.matia@surrey.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD002226

PRIDE project URI

• PRIDE
  1. PXD002226
     1. Label: PRIDE project
     2. Name: Caenorhabditis elegans mRBPome