PXD002221

PXD002221 is an original dataset announced via ProteomeXchange.

Title	mouse liver microsomes phosphorylation
Description	The microsomes are re-formed vesicle-like artifacts, not ordinarily present in living cells, from pieces of the endoplasmic reticulum (ER), plasma membrane, mitochondria, and Golgi apparatus of broken eukaryotic cells. Typically, microsomes are separated by differential centrifuged from homogenized of cell or tissue. Based on enrichment of ER fractions, prepared microsomes mainly show a large of metabolic activity following high amount of metabolic enzyme, as cytochrome P450 (CYP). CYPs are important components of xenobiotic-metabolizing monooxygenase accounting for an approximate 75% of biotransformation of clinically relevant drugs. Hepatic CYP are ER-anchored hemoproteins involved in the metabolism of numerous endo- and xenobiotics including drugs, steroids, and carcinogens. The CYPs catalytic activity is subject to regulation via phosphorylation. In several previous studies, 8 CYP phosphorylation sites had been discovered in human. Protein kinase A (PKA) and protein kinase C (PKC) were identified as a major catalyst for the phosphorylation of CYPs in 2003. The phosphorylation of CYPs process to their ubiquitination undergoing ubiquitin-dependent 26S proteosomal degradation.
HostingRepository	PRIDE
AnnounceDate	2015-08-24
AnnouncementXML	Submission_2015-08-24_02:53:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oh Kwang Kwon
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-22 08:48:35	ID requested
⏵ 1	2015-08-24 02:53:18	announced

Kwon OK, Lee W, Kim SJ, Lee YM, Lee JY, Kim JY, Bae JS, Lee S, In-depth proteomics approach of secretome to identify novel biomarker for sepsis in LPS-stimulated endothelial cells. Electrophoresis, 36(23):2851-8(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Mouse, Liver, Microsome, phosphorylation

Sangkyu Lee
contact affiliation	College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University
contact email	sangkyu@knu.ac.kr
lab head
Oh Kwang Kwon
contact affiliation	Kyungpook National University
contact email	kok0100@nate.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002221

PRIDE project URI

• PRIDE
  1. PXD002221
     1. Label: PRIDE project
     2. Name: mouse liver microsomes phosphorylation