PXD002218

PXD002218 is an original dataset announced via ProteomeXchange.

Title	Cytological and proteomic analyses of horsetail (Equisetum arvense) spore germination
Description	Spermatophyte pollen tubes and root hairs have been used as single-cell-type model systems toward understanding the molecular processes underlying polar growth of plant cells. Horsetail (Equisetum arvense L.) is a perennial herb species in Equisetopsida, which creates separately growing spring and summer stems in its life cycle. The mature chlorophyllous spores produced from spring stems can germinate without dormancy. Here we report the cellular features and protein expression patterns in five stages of horsetail spore germination (mature spores, rehydrated spores, double-celled spores, germinated spores, and spores with protonemal cells). Using 2-DE combined with mass spectrometry, 80 proteins were found to be differentially expressed upon spore germination. Among them, proteins involved in photosynthesis, protein turnover, and energy supply were over-represented. Thirteen proteins appeared as isoforms on the gels, indicating the potential importance of post-translational modification. In addition, the dynamic changes of ascorbate peroxidase, peroxiredoxin, and dehydroascorbate reductase implied that reactive oxygen species homeostasis is critical in regulating cell division and tip-growth. The time course of germination and diverse expression patterns of proteins in photosynthesis, energy supply, lipid and amino acid metabolism indicated that heterotrophic and autotrophic metabolism were necessary in light-dependent germination of the spores. Twenty-six proteins were involved in protein synthesis, folding, and degradation, indicating that protein turnover is vital to spore germination and rhizoid tip-growth. Furthermore, the altered abundance of small G protein Ran1, 14-3-3 protein, actin, and Caffeoyl-CoA O-methyltransferase revealed that signaling transduction, vesicle trafficking, cytoskeleton dynamics, and cell wall modulation were critical to cell division and polar growth. These findings provide up-to-date evidences for understanding fern spore asymmetric division and rhizoid polar growth.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_05:38:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shaojun Dai
SpeciesList	scientific name: Equisetum arvense (Field horsetail) (Common horsetail); NCBI TaxID: 3258;
ModificationList	No PTMs are included in the dataset
Instrument	Q TRAP; QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-22 08:11:00	ID requested
1	2016-12-23 06:40:58	announced
2	2017-10-24 03:39:30	announced	Updated project metadata.
⏵ 3	2017-10-24 05:38:10	announced	Updated project metadata.

Zhao Q, Gao J, Suo J, Chen S, Wang T, Dai S, Cytological and proteomic analyses of horsetail (Equisetum arvense L.) spore germination. Front Plant Sci, 6():441(2015) [pubmed]

curator keyword: Biological

submitter keyword: Spore germination, Equisetum arvense L., fern, proteomics, single cell, polar growth

Shaojun Dai
contact affiliation	Shanghai Normal University, China
contact email	735425617@qq.com
lab head
Shaojun Dai
contact affiliation	NEFU
contact email	735425617@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002218
     1. Label: PRIDE project
     2. Name: Cytological and proteomic analyses of horsetail (Equisetum arvense) spore germination