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PXD002206

PXD002206 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNovel non-canonical role of STAT1 in Natural Killer cell cytotoxicity
DescriptionSTAT1 is an important regulator of NK cell maturation and cytotoxicity. Although the consequences of Stat1-deficiency have been described in detail the underlying molecular functions of STAT1 in NK cells are only partially understood. Here we describe a novel non-canonical role of STAT1 that was unmasked in NK cells expressing Stat1-Y701F. This mutation prevents JAK-dependent phosphorylation, subsequent nuclear translocation and cytokine-induced transcriptional activity. As expected Stat1-Y701F mice displayed impaired NK cell maturation comparable to Stat1-/- animals. In contrast Stat1-Y701F NK cells exerted a significantly enhanced cytotoxicity in vitro and in vivo suggesting a so-far unknown cytoplasmic function. Using immunofluorescence technology we uncovered the recruitment of STAT1 to the immunological synapse during NK cell killing. A Stat1ind mouse expressing FLAG-tagged STAT1α was used to study the STAT1α interactome in NK cells. Mass spectrometry revealed that STAT1 directly binds proteins involved in cell junction formation and proteins associated to membrane or membrane-bound vesicles. We propose a novel function for STAT1 in the immunological synapse of NK cells regulating tumor surveillance and cytotoxicity.
HostingRepositoryPRIDE
AnnounceDate2016-10-07
AnnouncementXMLSubmission_2019-02-12_07:52:32.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD002206
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristopher Gerner
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListOxidation; Acetyl; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-05-20 02:11:24ID requested
12016-10-07 02:32:08announced
22019-02-12 07:52:35announcedUpdated publication reference for PubMed record(s): 27757297.
Publication List
Putz EM, Majoros A, Gotthardt D, Prchal-Murphy M, Zebedin-Brandl EM, Fux DA, Schlattl A, Schreiber RD, Carotta S, M, ü, ller M, Gerner C, Decker T, Sexl V, Novel non-canonical role of STAT1 in Natural Killer cell cytotoxicity. Oncoimmunology, 5(9):e1186314(2016) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: immunological synapse, interactome, proteome, F-actin, vesicle
Contact List
Christopher Gerner
contact affiliationUniversity of Vienna, Faculty of Chemistry, Department of Analytical Chemistry
contact emailchristopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliationUniversity of Vienna
contact emailchristopher.gerner@univie.ac.at
dataset submitter
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Dataset FTP location
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PRIDE project URI
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