PXD002206

PXD002206 is an original dataset announced via ProteomeXchange.

Title	Novel non-canonical role of STAT1 in Natural Killer cell cytotoxicity
Description	STAT1 is an important regulator of NK cell maturation and cytotoxicity. Although the consequences of Stat1-deficiency have been described in detail the underlying molecular functions of STAT1 in NK cells are only partially understood. Here we describe a novel non-canonical role of STAT1 that was unmasked in NK cells expressing Stat1-Y701F. This mutation prevents JAK-dependent phosphorylation, subsequent nuclear translocation and cytokine-induced transcriptional activity. As expected Stat1-Y701F mice displayed impaired NK cell maturation comparable to Stat1-/- animals. In contrast Stat1-Y701F NK cells exerted a significantly enhanced cytotoxicity in vitro and in vivo suggesting a so-far unknown cytoplasmic function. Using immunofluorescence technology we uncovered the recruitment of STAT1 to the immunological synapse during NK cell killing. A Stat1ind mouse expressing FLAG-tagged STAT1α was used to study the STAT1α interactome in NK cells. Mass spectrometry revealed that STAT1 directly binds proteins involved in cell junction formation and proteins associated to membrane or membrane-bound vesicles. We propose a novel function for STAT1 in the immunological synapse of NK cells regulating tumor surveillance and cytotoxicity.
HostingRepository	PRIDE
AnnounceDate	2016-10-07
AnnouncementXML	Submission_2019-02-12_07:52:32.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002206
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christopher Gerner
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-20 02:11:24	ID requested
1	2016-10-07 02:32:08	announced
⏵ 2	2019-02-12 07:52:35	announced	Updated publication reference for PubMed record(s): 27757297.

Putz EM, Majoros A, Gotthardt D, Prchal-Murphy M, Zebedin-Brandl EM, Fux DA, Schlattl A, Schreiber RD, Carotta S, M, ü, ller M, Gerner C, Decker T, Sexl V, Novel non-canonical role of STAT1 in Natural Killer cell cytotoxicity. Oncoimmunology, 5(9):e1186314(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: immunological synapse, interactome, proteome, F-actin, vesicle

Christopher Gerner
contact affiliation	University of Vienna, Faculty of Chemistry, Department of Analytical Chemistry
contact email	christopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliation	University of Vienna
contact email	christopher.gerner@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD002206

PRIDE project URI

• PRIDE
  1. PXD002206
     1. Label: PRIDE project
     2. Name: Novel non-canonical role of STAT1 in Natural Killer cell cytotoxicity