PXD002187

PXD002187 is an original dataset announced via ProteomeXchange.

Title	Identification of protein N-termini in Cyanophora paradoxa cyanelles: Transit peptide composition and sequence determinants for precursor maturation
Description	Glaucophytes, rhodophyta and chloroplastida represent the three evolutionary lineages that diverged after primary endosymbiosis. Comparative analyses between members of these three lineages are a rich source of information on ancestral plastid features. We analyzed the composition and the cleavage site of cyanelle transit peptides from the glaucophyte Cyanophora paradoxa by terminal amine labelling of substrates (TAILS), and compared their characteristics to those of representatives of the chloroplastida. Our data show that transit peptide architecture is similar between members of these two lineages. This entails a comparable modular structure, an overrepresentation of serine or alanine and similarities in the amino acid composition around the processing peptidase cleavage site. The most distinctive difference is the overrepresentation of phenylalanine in the N-terminal 1-10 amino acids of cyanelle transit peptides. A quantitative proteome analysis with periplasm-free cyanelles identified 42 out of 262 proteins without the N-terminal phenylalanine, suggesting that the requirement for phenylalanine in this position is not absolute. Proteins in this set are on average of low abundance, suggesting that either alternative import pathways are operating specifically for low abundance proteins or that the gene model annotation is incorrect for proteins with fewer EST sequences. We discuss these two possibilities and provide examples for both interpretations.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:24:53.359.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sacha Baginsky
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-15 01:44:31	ID requested
1	2016-12-20 15:28:21	announced
2	2017-10-24 02:48:25	announced	Updated project metadata.
⏵ 3	2024-10-22 04:24:58	announced	2024-10-22: Updated project metadata.

10.3389/fpls.2015.00559;

K, ö, hler D, Dobritzsch D, Hoehenwarter W, Helm S, Steiner JM, Baginsky S, Identification of protein N-termini in Cyanophora paradoxa cyanelles: transit peptide composition and sequence determinants for precursor maturation. Front Plant Sci, 6():559(2015) [pubmed]

curator keyword: Biological

submitter keyword: cyanophera paradoxa, MS, chloroplast transit peptide, TAILS

Sacha Baginsky
contact affiliation	Martin-Luther-Universität Halle-Wittenberg Institut für Biochemie und Biotechnologie Abteilung Pflanzenbiochemie
contact email	pflanzen@biochemtech.uni-halle.de
lab head
Sacha Baginsky
contact affiliation	Institut fuer Biochemie und Biotechnologie
contact email	pflanzen@biochemtech.uni-halle.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD002187

PRIDE project URI

• PRIDE
  1. PXD002187
     1. Label: PRIDE project
     2. Name: Identification of protein N-termini in Cyanophora paradoxa cyanelles: Transit peptide composition and sequence determinants for precursor maturation