PXD002186

PXD002186 is an original dataset announced via ProteomeXchange.

Title	Function of clpF in the clp protease complex
Description	The plant plastid Clp machinery comprises a hetero-oligomeric ClpPRT proteolytic core, ATP-dependent ClpC,D chaperones and an adaptor protein, ClpS1. ClpS1 directs a subset of substrates to the Clp protease-chaperone complex for degradation, but substrate recognition and delivery mechanisms are unknown. Here we describe ClpF, a novel chloroplast adaptor. ClpF is only found in photosynthetic eukaryotes and contains uvr/C and YccV domains and an N-terminal domain conserved across ClpF homologs. We demonstrate that ClpF acts together with ClpS1 in substrate delivery to plastid ClpC chaperones. The molecular domain interactions of ClpF to ClpS1 and the ClpC chaperones were mapped, and in vivo interactions between ClpF and the Clp substrate glutamate t-RNA reductase (GluTR1) are demonstrated. Quantitative proteomics identified subtle molecular phenotype in a ClpF null mutant and we show that GluTR1 degradation is delayed in ClpF and ClpS1 Arabidopsis null mutants.
HostingRepository	PRIDE
AnnounceDate	2019-04-09
AnnouncementXML	Submission_2019-04-09_05:19:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002186
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Giulia Friso
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; acetylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-14 05:12:51	ID requested
1	2019-04-09 05:09:02	announced
⏵ 2	2019-04-09 05:19:01	announced	Updated publication reference for PubMed record(s): 26419670.

Nishimura K, Apitz J, Friso G, Kim J, Ponnala L, Grimm B, van Wijk KJ, Discovery of a Unique Clp Component, ClpF, in Chloroplasts: A Proposed Binary ClpF-ClpS1 Adaptor Complex Functions in Substrate Recognition and Delivery. Plant Cell, 27(10):2677-91(2015) [pubmed]

curator keyword: Biological

submitter keyword: Plant cell, Arabidopsis thaliana, chloroplast, stroma, clp complex , coIP, LC-MSMS

Giulia Friso c/o Klaas van Wijk's Lab
contact affiliation	Cornell University, Ithaca, NY (USA)
contact email	gf32@cornell.edu
lab head
Giulia Friso
contact affiliation	Plant Biology
contact email	gf32@cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002186
     1. Label: PRIDE project
     2. Name: Function of clpF in the clp protease complex