PXD002185

PXD002185 is an original dataset announced via ProteomeXchange.

Title	Schistosoma japonicum cercaria and schistosomula
Description	Schistosomiasis is a parasitic zoonosis which caused by schistosomes and poses great threat to the health of human and other mammals. Schistosome cercaria must penetrate skin as an initial step to successfully infect the final host. Proteolytic enzymes secreted from the acetabular glands of cercaria contribute significantly to the invasion process. In this study, we designed a new cercaria transformation experiment to get the penetrated cercaria (schistosomula), then a gel-free shotgun proteomic analysis were performed to compare the proteomes of cercaria and schistosomula. 1894 and 1002 proteins were identified respectively, 924 proteins were common in both of two samples, almost take up 92% of the total proteins of schistosomula. The identified proteins were closely associated with nine main biological processes through Gene Ontology (GO) categorical analysis. The identification of the proteins potentially related to skin penetration offer a global overview of changes during cercaria skin invasion, providing clues into how they involve in the penetration process. Various proteases and peptidases were detected, and were differentially existed before and after cercaria transformation, suggesting proteases-specific roles and complexity mechanism during invasion.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:24:44.544.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mu Liu
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-14 02:14:20	ID requested
1	2017-05-08 02:10:42	announced
⏵ 2	2024-10-22 04:24:45	announced	2024-10-22: Updated project metadata.

Liu M, Ju C, Du XF, Shen HM, Wang JP, Li J, Zhang XM, Feng Z, Hu W, Proteomic Analysis on Cercariae and Schistosomula in Reference to Potential Proteases Involved in Host Invasion of Schistosoma japonicum Larvae. J Proteome Res, 14(11):4623-34(2015) [pubmed]

10.1021/acs.jproteome.5b00465;

curator keyword: Biological, Biomedical

submitter keyword: schistosomula, cercaria, proteome,schistosoma japonicum

Mu Liu
contact affiliation	Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China
contact email	lovemotherchina@163.com
lab head
Mu Liu
contact affiliation	Fudan university
contact email	lovemotherchina@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD002185

PRIDE project URI

• PRIDE
  1. PXD002185
     1. Label: PRIDE project
     2. Name: Schistosoma japonicum cercaria and schistosomula