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PXD002185

PXD002185 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSchistosoma japonicum cercaria and schistosomula
DescriptionSchistosomiasis is a parasitic zoonosis which caused by schistosomes and poses great threat to the health of human and other mammals. Schistosome cercaria must penetrate skin as an initial step to successfully infect the final host. Proteolytic enzymes secreted from the acetabular glands of cercaria contribute significantly to the invasion process. In this study, we designed a new cercaria transformation experiment to get the penetrated cercaria (schistosomula), then a gel-free shotgun proteomic analysis were performed to compare the proteomes of cercaria and schistosomula. 1894 and 1002 proteins were identified respectively, 924 proteins were common in both of two samples, almost take up 92% of the total proteins of schistosomula. The identified proteins were closely associated with nine main biological processes through Gene Ontology (GO) categorical analysis. The identification of the proteins potentially related to skin penetration offer a global overview of changes during cercaria skin invasion, providing clues into how they involve in the penetration process. Various proteases and peptidases were detected, and were differentially existed before and after cercaria transformation, suggesting proteases-specific roles and complexity mechanism during invasion.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:24:44.544.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMu Liu
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListmonohydroxylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-05-14 02:14:20ID requested
12017-05-08 02:10:42announced
22024-10-22 04:24:45announced2024-10-22: Updated project metadata.
Publication List
Liu M, Ju C, Du XF, Shen HM, Wang JP, Li J, Zhang XM, Feng Z, Hu W, Proteomic Analysis on Cercariae and Schistosomula in Reference to Potential Proteases Involved in Host Invasion of Schistosoma japonicum Larvae. J Proteome Res, 14(11):4623-34(2015) [pubmed]
10.1021/acs.jproteome.5b00465;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: schistosomula, cercaria, proteome,schistosoma japonicum
Contact List
Mu Liu
contact affiliationDepartment of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China
contact emaillovemotherchina@163.com
lab head
Mu Liu
contact affiliationFudan university
contact emaillovemotherchina@163.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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