PXD002181

PXD002181 is an original dataset announced via ProteomeXchange.

Title	Metabolic pathways affected by Anaplasma in tick cells
Description	Anaplasma phagocytophilum is an emerging zoonotic pathogen that causes human granulocytic anaplasmosis. These intracellular bacteria establish infection by affecting cell function in both the vertebrate host and the tick vector, Ixodes scapularis. Previous studies have characterized the tick transcriptome and proteome in response to A. phagocytophilum infection. However, in the post-genomic era, the integration of omics datasets through a systems biology approach allows network-based analyses to describe the complexity and functionality of biological systems such as host-pathogen interactions and the discovery of new targets for prevention and control of infectious diseases. This study reports for the first time a systems biology integration of metabolomics, transcriptomics and proteomics data to characterize essential metabolic pathways involved in the response of tick cells to A. phagocytophilum infection. The results showed that infection affected protein processing in endoplasmic reticulum and glucose metabolic pathways in tick cells. These results supported tick-Anaplasma co-evolution by providing new evidence of how tick cells limit pathogen infection, while the pathogen benefits from the tick cell response to establish infection. The results suggested that A. phagocytophilum induces protein misfolding to limit the tick cell response and facilitate infection, but requires protein degradation to prevent ER stress and cell apoptosis to survive in infected cells. Additionally, A. phagocytophilum may benefit from the tick cell’s ability to limit bacterial infection through PEPCK inhibition leading to decreased glucose metabolism, which also results in the inhibition of cell apoptosis that increases infection of tick cells. These results support the use of this experimental approach to systematically identify tick cell pathways and molecular mechanisms involved in tick-pathogen interactions.
HostingRepository	PRIDE
AnnounceDate	2015-10-05
AnnouncementXML	Submission_2015-10-05_06:13:04.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002181
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Margarita Villar
SpeciesList	scientific name: Ixodes scapularis (Black-legged tick) (Deer tick); NCBI TaxID: 6945;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-13 01:58:36	ID requested
⏵ 1	2015-10-05 06:13:05	announced

Villar M, Ayll, ó, n N, Alberdi P, Moreno A, Moreno M, Tobes R, Mateos-Hern, á, ndez L, Weisheit S, Bell-Sakyi L, de la Fuente J, Integrated Metabolomics, Transcriptomics and Proteomics Identifies Metabolic Pathways Affected by Anaplasma phagocytophilum Infection in Tick Cells. Mol Cell Proteomics, 14(12):3154-72(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Ixodes scapularis ISE6 cells, Anaplasma phagocytophilum, RP-LC-LIT-MS

Margarita Villar
contact affiliation	Proteomics Lab. SaBio research group. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM)
contact email	margaritam.villar@uclm.es
lab head
Margarita Villar
contact affiliation	SaBio (Sanidad y Biotecnología) Research Group. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM)
contact email	margaritam.villar@uclm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD002181

PRIDE project URI

• PRIDE
  1. PXD002181
     1. Label: PRIDE project
     2. Name: Metabolic pathways affected by Anaplasma in tick cells