PXD002171

PXD002171 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteome analysis of hepatocellular carcinoma tissue
Description	Hepatocellular carcinoma (HCC) is one of the most aggressive tumors and the treatment outcome of this disease is improved when the cancer is diagnosed at early stages. This requires biomarkers allowing for an accurate and early tumor diagnosis. To identify potential markers for such applications we performed a label-free proteome analysis using samples collected from 19 patients. We analyzed the data considering events known to take place in early events of HCC development such as abnormal regulation of Wnt/b-catenin and activation of receptor tyrosine kinases (RTKs). We expected that such analysis can lead to the identification of potential biomarkers for early-stage HCC diagnosis. 31 proteins functionally linked to downstream interactors of Wnt/b-catenin and parts of RTKs´-activated pathways Ras and JAK/STAT were selected for verification in a larger and independent cohort (n= 31) using selected (multiple) reaction monitoring (SRM/MRM).
HostingRepository	PRIDE
AnnounceDate	2016-04-13
AnnouncementXML	Submission_2016-04-13_00:31:20.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002171
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Wael Naboulsi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-12 08:10:27	ID requested
⏵ 1	2016-04-13 00:31:21	announced

Naboulsi W, Megger DA, Bracht T, Kohl M, Turewicz M, Eisenacher M, Voss DM, Schlaak JF, Hoffmann AC, Weber F, Baba HA, Meyer HE, Sitek B, Quantitative Tissue Proteomics Analysis Reveals Versican as Potential Biomarker for Early-Stage Hepatocellular Carcinoma. J Proteome Res, 15(1):38-47(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, tissue, Hepatocellular carcinoma, label-free proteomics, early diagnosis biomarker, targeted proteomics, multiple reaction monitoring, MRM, selected reaction monitoring, SRM, Bimarkers, Versican.

Barbara Sitek
contact affiliation	Medizinisches Proteom-Center, Ruhr-Universität Bochum, Germany
contact email	barbara.sitek@rub.de
lab head
Wael Naboulsi
contact affiliation	MPC
contact email	Wael.naboulsi@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD002171

PRIDE project URI

• PRIDE
  1. PXD002171
     1. Label: PRIDE project
     2. Name: Quantitative proteome analysis of hepatocellular carcinoma tissue