PXD002166

PXD002166 is an original dataset announced via ProteomeXchange.

Title	Peroxidase B6T173 (ZmPrx35) from insect-resistant maize (Zea mays, p84C3) kernels
Description	Peroxidase (POD) activity was found to be positively correlated with insect resistance in maize kernels. Thus, we purified active PODs from (Zea mays, p84C3) kernels and subjected to LC-MS/MS analyses. To verify the identified putative plant PODs, we produced them by recombinant expression in E. coli. Whereas B4FFK9 did not display in vitro activity, B6T173 (ZmPrx35) was active and also exhibits a heme cofactor. B6T173 accounts for about 80% of the POD activity in the kernel, according to densitometric evaluation.
HostingRepository	PRIDE
AnnounceDate	2019-02-12
AnnouncementXML	Submission_2019-02-12_07:50:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Winkler
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	monohydroxylated residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-12 02:07:36	ID requested
1	2015-08-21 02:32:57	announced
⏵ 2	2019-02-12 07:50:11	announced	Updated publication reference for PubMed record(s): 26379694.

L, ó, pez-Castillo LM, L, ó, pez-Arciniega JA, Guerrero-Rangel A, Vald, é, s-Rodr, í, guez S, Brieba LG, Garc, í, a-Lara S, Winkler R, Identification of B6T173 (ZmPrx35) as the prevailing peroxidase in highly insect-resistant maize (Zea mays, p84C3) kernels by activity-directed purification. Front Plant Sci, 6():670(2015) [pubmed]

curator keyword: Biological

submitter keyword: insect resistance, plant peroxidase, activity-directed proteomics

Robert Winkler
contact affiliation	CINVESTAV Unidad Irapuato Km. 9.6 Libramiento Norte Carr. Irapuato-León 36821 Irapuato Gto., México Tel.: +52-462-6239-635
contact email	robert.winkler@ira.cinvestav.mx
lab head
Robert Winkler
contact affiliation	CINVESTAV Unidad Irapuato
contact email	robert.winkler@bioprocess.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002166

PRIDE project URI

• PRIDE
  1. PXD002166
     1. Label: PRIDE project
     2. Name: Peroxidase B6T173 (ZmPrx35) from insect-resistant maize (Zea mays, p84C3) kernels