PXD002160

PXD002160 is an original dataset announced via ProteomeXchange.

Title	PsbS interacting proteins - PsbS interactions involved in the activation of energy dissipation in Arabidopsis
Description	PsbS Protein Interactions during Non-photochemical quenching The non-photochemical quenching of excitation energy (NPQ) describes a photoprotective mechanism in the antenna of PSII which dissipates excess excitation energy as heat at the level of 1Chl* and by that prevents the formation of singlet oxygen in PSII. Four different components contribute to NPQ, namely qT, qE, qZ and qI. Under saturating light conditions, the qE represents the dominant NPQ component; qE is based on a complex mechanism which strictly depends on the ΔpH across the thylakoid membrane, the PsbS protein and the xanthophyll zeaxanthin (Zx). The central role of PsbS in these processes is related to the function of PsbS as sensor of the lumen pH. However, the molecular basis of this central function and particularly the underlying interactions of PsbS with PSII antenna proteins that lead to energy quenching are largely unclear. In this work, we present an in vitro approach to identify protein transient interactions involving the PsbS protein during NPQ induction and relaxation in Arabidopsis thaliana, revealing its direct role in the formation of quenching sites in the antenna complexes of photosystem II.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:48.874.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-11 05:26:51	ID requested
1	2016-01-12 06:49:08	announced
2	2016-06-01 04:53:29	announced	Updated publication reference for DOI(s): http://dx.doi.org/10.1038/nplants.2015.225.
3	2016-06-15 00:17:53	announced	Updated publication reference for DOI(s): http://dx.doi.org/10.1038/nplants.2015.225.
4	2017-08-06 23:57:06	announced	Updated project metadata.
⏵ 5	2023-11-14 08:48:49	announced	2023-11-14: Updated project metadata.

Correa-Galvis V, Poschmann G, Melzer M, St, ü, hler K, Jahns P, PsbS interactions involved in the activation of energy dissipation in Arabidopsis. Nat Plants, 2():15225(2016) [pubmed]

curator keyword: Biological

submitter keyword: AP-MS, Arabidopsis,PsbS, LC-MS, thylakoid

Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory (MPL) Biologisch-Medizinisches Forschungszentrum (BMFZ) Heinrich-Heine-Universität Düsseldorf Germany
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Heinrich Heine Universität Düsseldorf
contact email	gereon.poschmann@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002160

PRIDE project URI

• PRIDE
  1. PXD002160
     1. Label: PRIDE project
     2. Name: PsbS interacting proteins - PsbS interactions involved in the activation of energy dissipation in Arabidopsis