PXD002157

PXD002157 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of the role of Snail1 in the differentiation of mesenchymal stem cell
Description	Snail effects on adipogenesis was proposed to be mediated, among others, by a lack of response to TGFβ (Batlle et al., 2012) or by an apparent inhibition of PPARγ and C/EBPα expression (Lee et al., 2013). Similar results were obtained in preventing the differentiation of bone marrow-derived murine mesenchymal stem cells (mMSC) to osteoblasts or adipocytes (Batlle et al, 2012). Still, the molecular mechanisms underlying the effect of Snail on MSCs differentiation and the blocking of adipogenesis were far from being established. We carried out an in-depth quantitative proteomic analysis of Snail-transfected cells to identify the cellular and molecular mechanisms controlled by Snail. We used isobaric labeling with tandem mass tags (TMT) (Dayon and Sanchez, 2012) for mMSCs analysis.
HostingRepository	PRIDE
AnnounceDate	2015-09-08
AnnouncementXML	Submission_2015-09-09_06:40:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alberto Pelaez Garcia
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-08 03:30:43	ID requested
⏵ 1	2015-09-09 06:40:43	announced

Pel, á, ez-Garc, í, a A, Barderas R, Batlle R, Vi, ñ, as-Castells R, Bartolom, é RA, Torres S, Mendes M, Lopez-Lucendo M, Mazzolini R, Bonilla F, Garc, í, a de Herreros A, Casal JI, A proteomic analysis reveals that Snail regulates the expression of the nuclear orphan receptor Nuclear Receptor Subfamily 2 Group F Member 6 (Nr2f6) and interleukin 17 (IL-17) to inhibit adipocyte differentiation. Mol Cell Proteomics, 14(2):303-15(2015) [pubmed]

Pel, á, ez-Garc, í, a A, Barderas R, Mendes M, Lopez-Lucendo M, Sanchez JC, Garc, í, a de Herreros A, Casal JI, Data from proteomic characterization of the role of Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation. Data Brief, 4():606-13(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Snail1, TMT-6plex, MSC, Orbitrap-Velos

Functional Proteomics
contact affiliation	CIB-CSIC (Spanish Research Council)
contact email	icasal@cib.csic.es
lab head
Alberto Pelaez Garcia
contact affiliation	CSIC SPANISH RESEARCH COUNCIL
contact email	apelaez@cib.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/09/PXD002157

PRIDE project URI

• PRIDE
  1. PXD002157
     1. Label: PRIDE project
     2. Name: Proteomic characterization of the role of Snail1 in the differentiation of mesenchymal stem cell