PXD002153

PXD002153 is an original dataset announced via ProteomeXchange.

Title	Proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during aging
Description	The age of mosquitoes is a crucial determinant of their susceptibility to infection, probability of survival to transmit pathogens and tolerance to insecticides. We investigated changes to the abundance of proteins found in heads and thoraces of Anopheles gambiae and Anopheles stephensi as they aged. Protein expression changes were assessed using two-dimensional difference gel electrophoresis and the identity of differentially expressed proteins was determined by using either matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry or capillary high-pressure liquid chromatography coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometer. Protein biomarkers were validated by quantitative Western blot analysis.
HostingRepository	PRIDE
AnnounceDate	2015-08-26
AnnouncementXML	Submission_2015-08-26_04:47:21.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002153
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Keyur Dave
SpeciesList	scientific name: Anopheles gambiae (African malaria mosquito); NCBI TaxID: 7165; scientific name: Anopheles stephensi (Asian malaria mosquito); NCBI TaxID: 30069;
ModificationList	deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap; ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-07 03:25:31	ID requested
⏵ 1	2015-08-26 04:47:22	announced

Sikulu MT, Monkman J, Dave KA, Hastie ML, Dale PE, Kitching RL, Killeen GF, Kay BH, Gorman JJ, Hugo LE, Proteomic changes occurring in the malaria mosquitoes Anopheles gambiae and Anopheles stephensi during aging. J Proteomics, 126():234-44(2015) [pubmed]

curator keyword: Biological

submitter keyword: Anopheles gambiae, Anopheles stephensi, mass spectrometry, proteomics, Orbitrap, MALDI, Ageing, Malaria, Mosquito

Jeffrey J. Gorman
contact affiliation	Protein Discovery Centre, QIMR Berghofer Medical Research Institute, Herston, Queensland, 4029, Australia.
contact email	jeffrey.gorman@qimrberghofer.edu.au
lab head
Keyur Dave
contact affiliation	QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia
contact email	keyur.dave@qimrberghofer.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002153

PRIDE project URI

• PRIDE
  1. PXD002153
     1. Label: PRIDE project
     2. Name: Proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during aging