PXD002145

PXD002145 is an original dataset announced via ProteomeXchange.

Title	Towards understanding male infertility after spinal cord injury through quantitative proteomics
Description	The study of male infertility after spinal cord injury (SCI) has enhanced the understanding of seminal plasma (SP) as an important regulator of spermatozoa function. However, the most important factors leading to the diminished sperm motility and viability observed in semen of men with SCI remained unknown. Thus, to explore SP related molecular mechanisms underlying infertility after SCI we used mass spectrometry-based quantitative proteomics to compare SP retrieved from SCI patients to normal controls. As a result, we present an in-depth characterization of the human SP proteome, identifying ~2,800 unique proteins, and describe, in detail, the differential proteome observed in SCI. Our analysis demonstrates that a hyper-activation of the immune system may influence some seminal processes, which probably are not triggered by microbial infection. Moreover, we show evidence of an important prostate gland functional failure, i.e. diminished abundance of metabolic enzymes related to ATP turnover, secreted via prostasomes and identify the main outcome related to this fact and that it is intrinsically linked to the low sperm motility in SCI. Together, our data suggest the molecular pathways hindering fertility in SCI and shed new light on other causes of male infertility.
HostingRepository	PRIDE
AnnounceDate	2016-02-01
AnnouncementXML	Submission_2016-02-01_11:13:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominic Helm
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2x(13)C: 4x(2)H labeled dimethylated residue; dimethylated residue
Instrument	LTQ Orbitrap XL ETD; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-06 03:04:23	ID requested
⏵ 1	2016-02-01 11:13:42	announced

da Silva BF, Meng C, Helm D, Pachl F, Schiller J, Ibrahim E, Lynne CM, Brackett NL, Bertolla RP, Kuster B, Towards Understanding Male Infertility After Spinal Cord Injury Using Quantitative Proteomics. Mol Cell Proteomics, 15(4):1424-34(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: human seminal plasma, male infertility, spinal cord injury, anejaculation, LC-MS/MS, quantitative proteomics

Dominic Helm
contact affiliation	Chair of Proteomics and Bioanalytics, TU Muenchen
contact email	Dominic.Helm@tum.de
lab head
Dominic Helm
contact affiliation	Chair of Proteomics and Bioanalytics, TU Muenchen
contact email	dominic.helm@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD002145

PRIDE project URI

• PRIDE
  1. PXD002145
     1. Label: PRIDE project
     2. Name: Towards understanding male infertility after spinal cord injury through quantitative proteomics