PXD002139

PXD002139 is an original dataset announced via ProteomeXchange.

Title	AP-MS of tissue-specific protein complexes in C. elegans
Description	We present a method for the tissue-specific purification of protein complexes from the nematode C. elegans, based on cell-type specific biotinylation. To purify proteins from specific C. elegans tissues, we adapted a system based on in vivo biotinylation of a protein of interest. In this approach, a protein of interest is tagged with the 15 amino acid Avi-tag, which can be biotinylated in vivo by the BirA biotin ligase from Escherichia coli. The biotinylated bait protein and any associated proteins are then purified with streptavidin-coated beads, and their identities are determined by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:23:48.698.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Teck Yew Low
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-05 04:08:22	ID requested
1	2016-07-18 04:06:16	announced
2	2016-08-15 02:07:32	announced	Updated publication reference for PubMed record(s): 27506200.
⏵ 3	2024-10-22 04:23:56	announced	2024-10-22: Updated project metadata.

10.1186/s12915-016-0286-x;

Waaijers S, Mu, ñ, oz J, Berends C, Ramalho JJ, Goerdayal SS, Low TY, Zoumaro-Djayoon AD, Hoffmann M, Koorman T, Tas RP, Harterink M, Seelk S, Kerver J, Hoogenraad CC, Bossinger O, Tursun B, van den Heuvel S, Heck AJ, Boxem M, A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large. BMC Biol, 14():66(2016) [pubmed]

ProteomeXchange project tag: PRIME-XS Project

curator keyword: Biological

submitter keyword: BirA biotin ligase, C. elegans,AP-MS

Albert J.R. Heck
contact affiliation	1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands. 2. Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, The Netherlands.
contact email	A.J.R.Heck@uu.nl
lab head
Teck Yew Low
contact affiliation	Utrecht Univeristy
contact email	lowteckyew@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002139

PRIDE project URI

• PRIDE
  1. PXD002139
     1. Label: PRIDE project
     2. Name: AP-MS of tissue-specific protein complexes in C. elegans