PXD002137

PXD002137 is an original dataset announced via ProteomeXchange.

Title	Proteome Landscapes of the Human Colorectal Mucosa, and its Adenoma and Cancer
Description	Adenomatous polyps in the colorectal tract are benign tumors with the potential to develop cancer. In this study we perform a large scale proteomic study of comparing the proteomes of colorectal enterocytes (N) and the cells of the adenoma (A) and cancer (C). Using formalin fixed and paraffin embedded clinical material, we identified on average 10,000 proteins per sample of the microdissected cells and established a quantitative protein repository of the disease. Statistical analysis revealed 2300, 1780, and 2161 significant alterations between N and A, C and A, and C and N, respectively. In this work we did not aim identification of novel biomarkers but focus on depiction of the proteome alterations underlying the disease. The extent of the assessed changes reflects a varied cell size, the composition of different subcellular components, and basic biological processes including the energy metabolism, plasma membrane transport, DNA replication and transcription.
HostingRepository	PRIDE
AnnounceDate	2015-08-10
AnnouncementXML	Submission_2015-08-10_17:00:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-05 03:41:26	ID requested
1	2015-08-10 02:48:02	announced
⏵ 2	2015-08-10 17:00:44	announced	Updated publication reference for PubMed record(s): 26245529.

Wi, ś, niewski JR, Du, ś, -Szachniewicz K, Ostasiewicz P, Zi, ó, ł, kowski P, Rakus D, Mann M, Absolute Proteome Analysis of Colorectal Mucosa, Adenoma, and Cancer Reveals Drastic Changes in Fatty Acid Metabolism and Plasma Membrane Transporters. J Proteome Res, 14(9):4005-18(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Colorectal cancer, Adenomatous polyposis coli, Enterocyte, Metabolism, FFPE-tissue, Quantitative proteomics, Absolute protein quantification, Total protein approach

Jacek R Wisniewski
contact affiliation	Max-Planck Institute of Biochemistry, Martinsried, Germany
contact email	jwisniew@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002137

PRIDE project URI

• PRIDE
  1. PXD002137
     1. Label: PRIDE project
     2. Name: Proteome Landscapes of the Human Colorectal Mucosa, and its Adenoma and Cancer