PXD002133

PXD002133 is an original dataset announced via ProteomeXchange.

Title	Identifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry
Description	Mass spectrometry‐based in vitro kinase screens play an essential role in the discovery of kinase substrates, however, many suffer from biological and technical noise or necessitate genetically‐altered enzymecofactor systems. We describe a method that combines stable γ‐[18O2]‐ATP with classical in vitro kinase assays within a contemporary quantitative proteomic workflow. Our approach improved detection of known substrates of the non‐receptor tyrosine kinase ABL1; and identified potential, new in vitro substrates.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:23:55.299.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andre Mueller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-01 02:11:06	ID requested
1	2018-10-17 07:59:22	announced
⏵ 2	2024-10-22 04:24:03	announced	2024-10-22: Updated project metadata.

M, ü, ller AC, Giambruno R, Wei, ß, er J, M, á, jek P, Hofer A, Bigenzahn JW, Superti-Furga G, Jessen HJ, Bennett KL, Identifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry. Sci Rep, 6():28107(2016) [pubmed]

10.1038/srep28107;

curator keyword: Biological

submitter keyword: human, kinase substrates

in vitro kinase assay

proteomics

mass spectrometry

heavy ATP

non receptor tyrosine kinase Abl1

Keiryn L. Bennett
contact affiliation	Mass Spectrometry Department CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences Vienna Austria
contact email	kbennett@cemm.oeaw.ac.at
lab head
Andre Mueller
contact affiliation	Research Center for Molecular Medicine of the Austrian Academy of Sciences
contact email	amueller@cemm.oeaw.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD002133

PRIDE project URI

• PRIDE
  1. PXD002133
     1. Label: PRIDE project
     2. Name: Identifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry