PXD002123

PXD002123 is an original dataset announced via ProteomeXchange.

Title	Pleiotropic effect of Francisella ABC transporter gene inactivation on production of glycoconjugates
Description	The present study deals with the cross-talk of polysaccharide biosynthesis pathways of a highly infectious intracellular pathogen Francisella tularensis, the causative agent of tularemia. We have studied the consequences of disrupting the FTS_1402 gene that lies within the putative polysaccharide locus FTT0789-FTT0800 on production of glycoconjugates and host-pathogen associated properties. Based on the homology to campylobacterial PglK flippase, FTS_1402 was predicted to play a role in glycosylation as a transporter of the nascent glycan across the inner membrane before its addition to target protein. Surprisingly, an inactivation of FTS_1402 gene had a pleiotropic effect upon production of multiple surface glycoconjugates. We show that FTS_1402 encoded protein is required for complete glycosylation but also for biogenesis of other surface exopolysaccharides such as lipopolysaccharide, capsule and/or capsule-like complex. All monitored glycoconjugates had preserved structures but were synthesized in lower amounts when compared to the highly virulent parental strain. Inactivation of FTS_1402 had an unexpectedly marked impact on virulence of the mutant, which however, provided protection against subsequent systemic challenge. With particular respect to protein glycosylation in the mutant and a wild type FSC200 strain, we here provide relevant MS data.
HostingRepository	PRIDE
AnnounceDate	2016-02-04
AnnouncementXML	Submission_2016-02-03_23:56:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lucie Balonova
SpeciesList	scientific name: Francisella tularensis subsp. holarctica FSC200; NCBI TaxID: 351581;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-29 07:48:08	ID requested
⏵ 1	2016-02-03 23:56:36	announced

Dankova V, Balonova L, Link M, Straskova A, Sheshko V, Stulik J, Inactivation of Francisella tularensis Gene Encoding Putative ABC Transporter Has a Pleiotropic Effect upon Production of Various Glycoconjugates. J Proteome Res, 15(2):510-24(2016) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Francisella, glycosylation, Q-Exactive

Jiri Stulik
contact affiliation	Department of Molecular Pathology and Biology Faculty of Military Health Sciences University of Defence Trebesska 1575 500 01 Hradec Kralove Czech Republic
contact email	jiri.stulik@unob.cz
lab head
Lucie Balonova
contact affiliation	University of Defence
contact email	lucie.balonova@unob.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002123
     1. Label: PRIDE project
     2. Name: Pleiotropic effect of Francisella ABC transporter gene inactivation on production of glycoconjugates