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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-03-13
  • AnnouncementXML: Submission_2018-03-13_03:23:59.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD002120
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Andrew Bottrill
  • Title: The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth
  • Description: PknB is an essential serine/threonine protein kinase required for mycobacterial cell division and cell wall biosynthesis. Here we demonstrate that over-expression of the external PknB_PASTA domain in mycobacteria results in delayed regrowth, accumulation of elongated bacteria and increased sensitivity to -lactam antibiotics. These changes are accompanied by altered production of certain enzymes involved in biosynthesis of cell wall as revealed by proteomics studies. The growth inhibition caused by over-expression of the PknB_PASTA domain is completely abolished by enhanced concentration of magnesium ions but not muropeptides. Finally, we show that the addition of recombinant PASTA domain could prevent regrowth of Mycobacterium tuberculosis and therefore offers an alternative opportunity to control replication of this pathogen. These results suggest that the PknB_PASTA domain is involved in regulation of peptidoglycan biosynthesis and maintenance of cell wall architecture.
  • SpeciesList: scientific name: Mycobacterium smegmatis; NCBI TaxID: 1772;
  • ModificationList: Ammonia-loss; Dehydrated; TMT6plex; Oxidation; Acetyl; Carbamidomethyl
  • Instrument: LTQ Orbitrap Velos

Dataset History

VersionDatetimeStatusChangeLog Entry
02015-04-29 02:16:49ID requested
12018-03-13 03:24:00announced

Publication List

  1. Turapov O, Loraine J, Jenkins CH, Barthe P, McFeely D, Forti F, Ghisotti D, Hesek D, Lee M, Bottrill AR, Vollmer W, Mobashery S, Cohen-Gonsaud M, Mukamolova GV, The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth. Open Biol, 5(7):150025(2015) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Mycobacterium tuberculosis, serine/threonine protein kinase, magnesium, muropeptides, PknB, PASTA domain

Contact List

    Galina V. Mukamolova
    • contact affiliation: Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, LE1 9HN
    • contact email: gvm4@le.ac.uk
    • lab head:
    Andrew Bottrill
    • contact affiliation: University of Leicester
    • contact email: arb29@le.ac.uk
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List

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