PXD002120

PXD002120 is an original dataset announced via ProteomeXchange.

Title	The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth
Description	PknB is an essential serine/threonine protein kinase required for mycobacterial cell division and cell wall biosynthesis. Here we demonstrate that over-expression of the external PknB_PASTA domain in mycobacteria results in delayed regrowth, accumulation of elongated bacteria and increased sensitivity to -lactam antibiotics. These changes are accompanied by altered production of certain enzymes involved in biosynthesis of cell wall as revealed by proteomics studies. The growth inhibition caused by over-expression of the PknB_PASTA domain is completely abolished by enhanced concentration of magnesium ions but not muropeptides. Finally, we show that the addition of recombinant PASTA domain could prevent regrowth of Mycobacterium tuberculosis and therefore offers an alternative opportunity to control replication of this pathogen. These results suggest that the PknB_PASTA domain is involved in regulation of peptidoglycan biosynthesis and maintenance of cell wall architecture.
HostingRepository	PRIDE
AnnounceDate	2018-03-13
AnnouncementXML	Submission_2018-03-13_03:23:59.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002120
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Mycobacterium smegmatis; NCBI TaxID: 1772;
ModificationList	Ammonia-loss; Dehydrated; TMT6plex; Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-29 02:16:49	ID requested
⏵ 1	2018-03-13 03:24:00	announced

Turapov O, Loraine J, Jenkins CH, Barthe P, McFeely D, Forti F, Ghisotti D, Hesek D, Lee M, Bottrill AR, Vollmer W, Mobashery S, Cohen-Gonsaud M, Mukamolova GV, The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth. Open Biol, 5(7):150025(2015) [pubmed]

curator keyword: Biological

submitter keyword: Mycobacterium tuberculosis, serine/threonine protein kinase, magnesium, muropeptides, PknB, PASTA domain

Galina V. Mukamolova
contact affiliation	Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, LE1 9HN
contact email	gvm4@le.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Leicester
contact email	arb29@le.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002120
     1. Label: PRIDE project
     2. Name: The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth