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PXD002120

PXD002120 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth
DescriptionPknB is an essential serine/threonine protein kinase required for mycobacterial cell division and cell wall biosynthesis. Here we demonstrate that over-expression of the external PknB_PASTA domain in mycobacteria results in delayed regrowth, accumulation of elongated bacteria and increased sensitivity to -lactam antibiotics. These changes are accompanied by altered production of certain enzymes involved in biosynthesis of cell wall as revealed by proteomics studies. The growth inhibition caused by over-expression of the PknB_PASTA domain is completely abolished by enhanced concentration of magnesium ions but not muropeptides. Finally, we show that the addition of recombinant PASTA domain could prevent regrowth of Mycobacterium tuberculosis and therefore offers an alternative opportunity to control replication of this pathogen. These results suggest that the PknB_PASTA domain is involved in regulation of peptidoglycan biosynthesis and maintenance of cell wall architecture.
HostingRepositoryPRIDE
AnnounceDate2018-03-13
AnnouncementXMLSubmission_2018-03-13_03:23:59.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD002120
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAndrew Bottrill
SpeciesList scientific name: Mycobacterium smegmatis; NCBI TaxID: 1772;
ModificationListAmmonia-loss; Dehydrated; TMT6plex; Oxidation; Acetyl; Carbamidomethyl
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-04-29 02:16:49ID requested
12018-03-13 03:24:00announced
Publication List
Turapov O, Loraine J, Jenkins CH, Barthe P, McFeely D, Forti F, Ghisotti D, Hesek D, Lee M, Bottrill AR, Vollmer W, Mobashery S, Cohen-Gonsaud M, Mukamolova GV, The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth. Open Biol, 5(7):150025(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Mycobacterium tuberculosis, serine/threonine protein kinase, magnesium, muropeptides, PknB, PASTA domain
Contact List
Galina V. Mukamolova
contact affiliationDepartment of Infection, Immunity and Inflammation, University of Leicester, Leicester, LE1 9HN
contact emailgvm4@le.ac.uk
lab head
Andrew Bottrill
contact affiliationUniversity of Leicester
contact emailarb29@le.ac.uk
dataset submitter
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Dataset FTP location
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