PXD002119

PXD002119 is an original dataset announced via ProteomeXchange.

Title	Fat BodFat Body, Hungry Brain: Nutriproteomics in the Hypothalamus from a Mouse Model with Obesity and Anorexiay, Hungry Brain: Nutriproteomics in the Hypothalamus from a Mouse Model with Obesity and Anorexia
Description	Systemic acute inflammatory signals can cause profound anorexia by disrupting the physiological appetite regulation in the hypothalamic milieu. Conversely, obesity related chronic inflammation of the hypothalamus can disturb anorexigenic signals and promote abnormal body weight control. The aim of the present study was to compare the global hypothalamic endophenotype in C57/Bl6 mice exposed to a high-fat diet or with acute illness mediated by LPS. Ten-week old male C57/Bl6 mice (n=18) were randomly divided into four groups; the control 1 group (n =3) was fed a normal diet whereas the high-fat diet (HFD) group (n =6) was fed a high-fat diet for eight weeks. The control 2 group (n=3) received an intraperitoneal injection of saline whereas the LPS group (n=6) received an intraperitoneal injection of LPS. Mice were sacrificed 18-hr post-injection. Both control 2 and LPS groups were fed a normal diet for eight weeks before the injection. The hypothalamic regions were removed and analysed using a 2D LC-MS methodology. The proteomic analysis profiled 9,235 proteins (q<0.05) across all biological states, of which 522 proteins were found modulated in the HFD group and another 579 in the LPS group. The proteomic profiles demonstrated that the systemic acute inflammation linked with anorexia induced a negative feedback loop of appetite control in the hypothalamus, suggesting an effort to re-establish homeostasis. By contrast, the chronic inflammation associated with obesity initiated a “perpetual cycle” of positive feedback enhancement of appetite regulation further exacerbating positive energy balance.
HostingRepository	PRIDE
AnnounceDate	2018-01-29
AnnouncementXML	Submission_2018-01-31_00:32:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harvey Johnston
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; deaminated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-29 02:05:52	ID requested
⏵ 1	2018-01-31 00:32:29	announced

Manousopoulou A, Koutmani Y, Karaliota S, Woelk CH, Manolakos ES, Karalis K, Garbis SD, Hypothalamus proteomics from mouse models with obesity and anorexia reveals therapeutic targets of appetite regulation. Nutr Diabetes, 6(4):e204(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: proteomics, hypothalamus, obesity, anorexia, acute inflammation, chronic inflammation

Spiros D. Garbis
contact affiliation	Cancer Sciences Unit, Faculty of Medicine, University of Southampton, Southampton, UK
contact email	s.d.garbis@soton.ac.uk
lab head
Harvey Johnston
contact affiliation	University of Southampton
contact email	h.johnston@soton.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002119
     1. Label: PRIDE project
     2. Name: Fat BodFat Body, Hungry Brain: Nutriproteomics in the Hypothalamus from a Mouse Model with Obesity and Anorexiay, Hungry Brain: Nutriproteomics in the Hypothalamus from a Mouse Model with Obesity and Anorexia