PXD002117

PXD002117 is an original dataset announced via ProteomeXchange.

Title	The quantitative proteome of a human cell line
Description	The generation of mathematical models of biological processes, the simulation of these processes under different conditions, and the comparison and integration of multiple data sets are explicit goals of systems biology that require the knowledge of the absolute quantity of the system's components. To date, systematic estimates of cellular protein concentrations have been exceptionally scarce. Here, we provide a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis. We show that these human cultured cells express at least ∼10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell. We discuss how protein abundance is linked to function and evolution.
HostingRepository	PRIDE
AnnounceDate	2015-05-07
AnnouncementXML	Submission_2015-05-07_02:11:59.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002117
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap; LTQ FT; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-29 01:26:30	ID requested
⏵ 1	2015-05-07 02:12:02	announced

Beck M, Schmidt A, Malmstroem J, Claassen M, Ori A, Szymborska A, Herzog F, Rinner O, Ellenberg J, Aebersold R, The quantitative proteome of a human cell line. Mol Syst Biol, 7():549(2011) [pubmed]

curator keyword: Biological

submitter keyword: mass spectrometry, absolute quantification, U2OS, Nuclear Pore Complex

Alexander Schmidt
contact affiliation	Biozentrum, University of Basel
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002117
     1. Label: PRIDE project
     2. Name: The quantitative proteome of a human cell line