PXD002117 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The quantitative proteome of a human cell line |
Description | The generation of mathematical models of biological processes, the simulation of these processes under different conditions, and the comparison and integration of multiple data sets are explicit goals of systems biology that require the knowledge of the absolute quantity of the system's components. To date, systematic estimates of cellular protein concentrations have been exceptionally scarce. Here, we provide a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis. We show that these human cultured cells express at least ∼10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell. We discuss how protein abundance is linked to function and evolution. |
HostingRepository | PRIDE |
AnnounceDate | 2015-05-07 |
AnnouncementXML | Submission_2015-05-07_02:11:59.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD002117 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alexander Schmidt |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Oxidation; Acetyl; Carbamidomethyl |
Instrument | LTQ Orbitrap; LTQ FT; LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-04-29 01:26:30 | ID requested | |
⏵ 1 | 2015-05-07 02:12:02 | announced | |
Publication List
Beck M, Schmidt A, Malmstroem J, Claassen M, Ori A, Szymborska A, Herzog F, Rinner O, Ellenberg J, Aebersold R, The quantitative proteome of a human cell line. Mol Syst Biol, 7():549(2011) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: mass spectrometry, absolute quantification, U2OS, Nuclear Pore Complex |
Contact List
Alexander Schmidt |
contact affiliation | Biozentrum, University of Basel |
contact email | alex.schmidt@unibas.ch |
lab head | |
Alexander Schmidt |
contact affiliation | Proteomics Core Facility |
contact email | alex.schmidt@unibas.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/05/PXD002117 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002117
- Label: PRIDE project
- Name: The quantitative proteome of a human cell line