PXD002116

PXD002116 is an original dataset announced via ProteomeXchange.

Title	Trace-level concentrations of metconazole and isoproturon induce a metabolic stress in Pacific oyster
Description	The Pacific oyster Crassostrea gigas is a sessile animal that undergoes highly dynamic and stressful environmental constraints all along its life in the intertidal zone and estuaries. We analyzed the physiological effects induced by low environmental concentrations of two pesticides, metconazole (0.2 and 2 µg L-1) or isoproturon (0.1 and 1 µg L-1), or in mix (0.2 and 0.1 µg L-1 respectively) known to be encountered by oysters in the field. At such trace-level, metconazole and isoproturon induced a metabolic stress, reflected by an important over-activation of the sensing-kinase AMP-activated kinase  (AMPK). Such deregulation reflect alteration of energy management, since the sensing-molecule AMPK plays a key role in C. gigas energetic metabolism, as well as alteration in the adaptation to environmental stress, given its role in hypoxia. Neither pesticide had any effect on eco-physiological parameters during the 14 days-experiment: filtration rate, food consumption, growth, and available energy reserves remained unchanged. In contrast, using two-dimensional electrophoresis, we identified changes in proteins that belong to energetic metabolism and cytoskeletal induced by metconazole or isoproturon. Analyses of antioxidant enzymes revealed an increase in oxidative stress pathway in superoxide dismutase and catalase in some conditions. Taken together, our results proved that trace-level environmental concentrations of metconazole or isoproturon provoke important deleterious effects at the biochemical level in oyster, by inducing a metabolic stress and oxidative stress response. Such alterations were not directly reflected at the level of the whole organism when measuring eco-physiological parameters. Identifying the impacts of pesticides at environmental concentrations using biochemical approaches is thus a promising field of research to improve monitoring processes.
HostingRepository	PRIDE
AnnounceDate	2022-03-01
AnnouncementXML	Submission_2022-03-01_03:17:42.480.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melanie Lagarrigue
SpeciesList	scientific name: Crassostrea gigas (Pacific oyster) (Crassostrea angulata); NCBI TaxID: 29159;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-29 01:22:06	ID requested
⏵ 1	2022-03-01 03:17:43	announced

Epelboin Y, Qu, é, r, é C, Pernet F, Pichereau V, Corporeau C, Energy and antioxidant responses of pacific oyster exposed to trace levels of pesticides. Chem Res Toxicol, 28(9):1831-41(2015) [pubmed]

curator keyword: Biological

submitter keyword: trace-level, pesticides, Marine bivalves, Crassostrea gigas, metabolism

Charles Pineau
contact affiliation	Protim - Irset Inserm U1085
contact email	charles.pineau@inserm.fr
lab head
Melanie Lagarrigue
contact affiliation	Life Sciences
contact email	melanie.lagarrigue@univ-rennes1.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002116
     1. Label: PRIDE project
     2. Name: Trace-level concentrations of metconazole and isoproturon induce a metabolic stress in Pacific oyster