PXD002115

PXD002115 is an original dataset announced via ProteomeXchange.

Title	Proteomic map of the unsequenced kala-azar vector Phlebotomus papatasi
Description	The debilitating disease kala-azar or visceral leishmaniasis (VL) is caused by the kinetoplastid protozoan parasite Leishmania donovani. The parasite is transmitted by the hematophagous sandfly vector of the genus Phlebotomus in the old world and Lutzomyia in the new world. The predominant Phlebotomine species associated with transmission of kala-azar are Phlebotomus papatasi and Phlebotomus argentipes. The infected female sandfly transmits the parasite when it takes a blood meal. Understanding the molecular interaction of the sand fly-Leishmania during the development of parasite within the gut of the sandfly is crucial to understanding parasite life cycle. The complete genome sequences of sandfly vectors (Phlebotomus and Lutzomyia) are currently not available and sequencing efforts are underway. Non-availability of genome sequence can hamper identification of proteins in the sandfly vector. In the present study we have carried out proteogenomic analysis of unsequenced sandfly vector P. paptasi cell line using high-resolution mass spectrometry and comparative homology-based searches using related dipteran protein data (mosquitoes and fruit fly). This study resulted in identification of 1,312 proteins from P. papatasi based on homology. Our study demonstrates the power of proteogenomic approaches in mapping the proteomes of unsequenced organisms.
HostingRepository	PRIDE
AnnounceDate	2015-08-31
AnnouncementXML	Submission_2015-08-31_02:38:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akhilesh Pandey
SpeciesList	scientific name: Phlebotomus papatasi (Sandfly); NCBI TaxID: 29031;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-28 08:56:11	ID requested
⏵ 1	2015-08-31 02:38:38	announced

Pawar H, Chavan S, Mahale K, Khobragade S, Kulkarni A, Patil A, Chaphekar D, Varriar P, Sudeep A, Pai K, Prasad TSK, Gowda H, Patole MS, A proteomic map of the unsequenced kala-azar vector Phlebotomus papatasi using cell line. Acta Trop, 152():80-89(2015) [pubmed]

curator keyword: Biological

submitter keyword: comparative proteogenomics, hematophagous arthropod and parasite vector

Akhilesh Pandey
contact affiliation	McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 USA
contact email	pandey@jhmi.edu
lab head
Akhilesh Pandey
contact affiliation	Johns Hopkins University
contact email	pandey@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002115
     1. Label: PRIDE project
     2. Name: Proteomic map of the unsequenced kala-azar vector Phlebotomus papatasi