PXD002113 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | C. trachomatis L2 inclusion proteome |
Description | Chlamydia trachomatis is an important human pathogen that replicates inside the infected host cell in a unique vacuole, the inclusion. The formation of this intracellular bacterial niche is essential for productive Chlamydia infections. Despite its importance for Chlamydia biology, a holistic view on the protein composition of the inclusion, including its membrane, is currently missing. Here we describe a newly established method to purify inclusions from C. trachomatis infected epithelial cells and the analysis of the host cell-derived proteome by a combination of label free and stable isotope labeling -based quantitative proteomics. Computational analysis of the proteome data indicated that the inclusion is a complex intracellular trafficking platform that interacts with host cells' antero- and retrograde trafficking pathways. Furthermore, the inclusion is highly enriched for sorting nexins of the SNX-BAR retromer, a complex essential for retrograde trafficking. Functional studies showed that in particular SNX5 controls the C. trachomatis infection and that retrograde trafficking is essential for infectious progeny formation. In summary, our findings suggest that the inclusion of C. trachomatis is well embedded in the hosts' endomembrane system and hijacks retrograde trafficking pathways for effective infection. |
HostingRepository | PRIDE |
AnnounceDate | 2015-06-08 |
AnnouncementXML | Submission_2015-06-08_07:40:23.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lukas Aeberhard |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-04-28 02:08:39 | ID requested | |
⏵ 1 | 2015-06-08 07:40:25 | announced | |
Publication List
Aeberhard L, Banhart S, Fischer M, Jehmlich N, Rose L, Koch S, Laue M, Renard BY, Schmidt F, Heuer D, The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components. PLoS Pathog, 11(6):e1004883(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Chlamydia, trachomatis, inclusion, vacuole, compartment, LC-MS/MS |
Contact List
Dagmar Heuer |
contact affiliation | Junior Research Group `Sexually Transmitted Bacterial Pathogens�, Robert Koch Institute, Berlin, Germany |
contact email | heuerd@rki.de |
lab head | |
Lukas Aeberhard |
contact affiliation | Robert Koch Institut |
contact email | lukas.aeberhard@gmx.net |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/06/PXD002113 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002113
- Label: PRIDE project
- Name: C. trachomatis L2 inclusion proteome