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PXD002113

PXD002113 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleC. trachomatis L2 inclusion proteome
DescriptionChlamydia trachomatis is an important human pathogen that replicates inside the infected host cell in a unique vacuole, the inclusion. The formation of this intracellular bacterial niche is essential for productive Chlamydia infections. Despite its importance for Chlamydia biology, a holistic view on the protein composition of the inclusion, including its membrane, is currently missing. Here we describe a newly established method to purify inclusions from C. trachomatis infected epithelial cells and the analysis of the host cell-derived proteome by a combination of label free and stable isotope labeling -based quantitative proteomics. Computational analysis of the proteome data indicated that the inclusion is a complex intracellular trafficking platform that interacts with host cells' antero- and retrograde trafficking pathways. Furthermore, the inclusion is highly enriched for sorting nexins of the SNX-BAR retromer, a complex essential for retrograde trafficking. Functional studies showed that in particular SNX5 controls the C. trachomatis infection and that retrograde trafficking is essential for infectious progeny formation. In summary, our findings suggest that the inclusion of C. trachomatis is well embedded in the hosts' endomembrane system and hijacks retrograde trafficking pathways for effective infection.
HostingRepositoryPRIDE
AnnounceDate2015-06-08
AnnouncementXMLSubmission_2015-06-08_07:40:23.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLukas Aeberhard
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-04-28 02:08:39ID requested
12015-06-08 07:40:25announced
Publication List
Aeberhard L, Banhart S, Fischer M, Jehmlich N, Rose L, Koch S, Laue M, Renard BY, Schmidt F, Heuer D, The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components. PLoS Pathog, 11(6):e1004883(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Chlamydia, trachomatis, inclusion, vacuole, compartment, LC-MS/MS
Contact List
Dagmar Heuer
contact affiliationJunior Research Group `Sexually Transmitted Bacterial Pathogens�, Robert Koch Institute, Berlin, Germany
contact emailheuerd@rki.de
lab head
Lukas Aeberhard
contact affiliationRobert Koch Institut
contact emaillukas.aeberhard@gmx.net
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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