PXD002100

PXD002100 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians
Description	The planarian Dugesia japonica has amazing ability to regenerate a head from the anterior ends of the amputated stump with maintenance of the original anterior-posterior polarity. Although planarians present an attractive system for molecular investigation of regeneration and research has focused on clarifying the molecular mechanism of regeneration initiation in planarians, but proteomic studies are still in the early stages. Here, a global proteomics analysis of regenerating head fragments in planarians at 0h, 2h and 6h after amputation was performed using isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics coupled with LC-MS/MS strategy. Then the significantly changed proteins were identified. The correlation betwwen protein expression profiles and signaling pathways/biological processes was analyzed by bioinformatics and systems biology.
HostingRepository	PRIDE
AnnounceDate	2015-07-07
AnnouncementXML	Submission_2015-07-07_09:58:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	cunshuan xu
SpeciesList	scientific name: Dugesia japonica (Planarian); NCBI TaxID: 6161;
ModificationList	monohydroxylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-24 04:08:52	ID requested
1	2015-07-07 06:10:39	announced
⏵ 2	2015-07-07 09:58:14	announced	Updated publication reference for PubMed record(s): 26142280, 26131905.

Swaney DL, Rodr, í, guez-Mias RA, Vill, é, n J, Phosphorylation of ubiquitin at Ser65 affects its polymerization, targets, and proteome-wide turnover. EMBO Rep, 16(9):1131-44(2015) [pubmed]

Geng X, Wang G, Qin Y, Zang X, Li P, Geng Z, Xue D, Dong Z, Ma K, Chen G, Xu C, iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians. PLoS One, 10(7):e0132045(2015) [pubmed]

curator keyword: Biological

submitter keyword: Planarian, Regenerating head fragments, iTRAQ, LC-MS/MS

Cunshuan Xu
contact affiliation	State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, Henan Province, China
contact email	cellkeylab@126.com
lab head
cunshuan xu
contact affiliation	henan normal university
contact email	cellkeylab@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002100
     1. Label: PRIDE project
     2. Name: iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians