PXD002079

PXD002079 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis of cell-based resistance to BRAF inhibitor therapy in melanoma
Description	The treatment of melanoma by targeted inhibition of the mutated kinase BRAF with small molecules only temporarily suppresses metastatic disease. In the face of chemical inhibition tumor plasticity, both innate and adaptive, promotes survival through the biochemical and genetic reconfiguration of cellular pathways that can engage proliferative and migratory systems. To investigate this process high-resolution mass spectrometry was used to characterize the phosphoproteome of this transition in vitro. A simple and accurate, label-free quantitative method was used to localize and quantitate thousands of phosphorylation events. We also correlated changes in the phosphoproteome with the proteome to more accurately determine changes in the activity of regulatory kinases determined by kinase landscape profiling. The abundance of phosphopeptides with sites that function in cytoskeletal regulation, GTP/GDP exchange, Protein Kinase C, IGF signaling and melanosome maturation were highly divergent after transition to a drug resistant phenotype.
HostingRepository	PRIDE
AnnounceDate	2018-10-24
AnnouncementXML	Submission_2018-10-24_09:06:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Parker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-20 01:26:44	ID requested
⏵ 1	2018-10-24 09:06:48	announced

Parker R, Vella LJ, Xavier D, Amirkhani A, Parker J, Cebon J, Molloy MP, Phosphoproteomic Analysis of Cell-Based Resistance to BRAF Inhibitor Therapy in Melanoma. Front Oncol, 5():95(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Phosphoproteomics, BRAF, Drug resistance, Kinases, Label free quantitation, Mass spectrometry

Mark Molloy
contact affiliation	Associate Professor APAF Director Australian Proteome Analysis Facility (APAF) Dept. Chemistry and Biomolecular Sciences E8C310, Research Park Drive Macquarie University Sydney NSW 2109 Australia Secretary, Australasian Proteomics Society
contact email	MMolloy@proteome.org.au
lab head
Robert Parker
contact affiliation	Centre for High Throughput Biology
contact email	robert.rparker@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD002079

PRIDE project URI

• PRIDE
  1. PXD002079
     1. Label: PRIDE project
     2. Name: Phosphoproteomic analysis of cell-based resistance to BRAF inhibitor therapy in melanoma