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PXD002076

PXD002076 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA piRNA like small RNA interacts with and modulates p-ERM proteins in human somatic cells
DescriptionPIWI-interacting RNAs (piRNAs) are thought to silence transposon and gene expression during development. However, the roles of piRNAs in somatic tissues are largely unknown. Here we report the identification of 555 piRNAs in human lung bronchial epithelial (HBE) and non-small cell lung cancer (NSCLC) cell lines, including 295 that don’t exist in databases termed as piRNA-Like sncRNAs or piRNA-Ls. Distinctive piRNA/piRNA-L expression patterns are observed between HBE and NSCLC cells. piRNA-L-163 (piR-L-163), the top down-regulated piRNA-L in NSCLC cells, binds directly to phosphorylated ERM proteins (p-ERM), which is dependent on the central part of UUNNUUUNNUU motif in piR-L-163 and the RRRKPDT element in ERM, and. The piR-L-163/p-ERM interaction is critical for p-ERM’s binding capability to filamentous actin (F-actin) and ERM-binding phosphoprotein 50 (EBP50). Thus, piRNA/piRNA-L may play a regulatory role through direct interaction with proteins in physiological and pathophysiological conditions.
HostingRepositoryPRIDE
AnnounceDate2015-09-03
AnnouncementXMLSubmission_2015-09-03_02:48:21.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDavid Clark
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-04-17 01:53:12ID requested
12015-09-03 01:29:26announced
22015-09-03 02:48:23announcedUpdated project metadata.
Publication List
Mei Y, Wang Y, Kumari P, Shetty AC, Clark D, Gable T, MacKerell AD, Ma MZ, Weber DJ, Yang AJ, Edelman MJ, Mao L, A piRNA-like small RNA interacts with and modulates p-ERM proteins in human somatic cells. Nat Commun, 6():7316(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Human, pull-down, LC-MS/MS
Contact List
Li Mao
contact affiliationDepartment of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, Baltimore MD
contact emailLMao@umaryland.edu
lab head
David Clark
contact affiliationUMB School of Dentistry
contact emaildavid.clark@umaryland.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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