PXD002076 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A piRNA like small RNA interacts with and modulates p-ERM proteins in human somatic cells |
Description | PIWI-interacting RNAs (piRNAs) are thought to silence transposon and gene expression during development. However, the roles of piRNAs in somatic tissues are largely unknown. Here we report the identification of 555 piRNAs in human lung bronchial epithelial (HBE) and non-small cell lung cancer (NSCLC) cell lines, including 295 that don’t exist in databases termed as piRNA-Like sncRNAs or piRNA-Ls. Distinctive piRNA/piRNA-L expression patterns are observed between HBE and NSCLC cells. piRNA-L-163 (piR-L-163), the top down-regulated piRNA-L in NSCLC cells, binds directly to phosphorylated ERM proteins (p-ERM), which is dependent on the central part of UUNNUUUNNUU motif in piR-L-163 and the RRRKPDT element in ERM, and. The piR-L-163/p-ERM interaction is critical for p-ERM’s binding capability to filamentous actin (F-actin) and ERM-binding phosphoprotein 50 (EBP50). Thus, piRNA/piRNA-L may play a regulatory role through direct interaction with proteins in physiological and pathophysiological conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2015-09-03 |
AnnouncementXML | Submission_2015-09-03_02:48:21.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | David Clark |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-04-17 01:53:12 | ID requested | |
1 | 2015-09-03 01:29:26 | announced | |
⏵ 2 | 2015-09-03 02:48:23 | announced | Updated project metadata. |
Publication List
Mei Y, Wang Y, Kumari P, Shetty AC, Clark D, Gable T, MacKerell AD, Ma MZ, Weber DJ, Yang AJ, Edelman MJ, Mao L, A piRNA-like small RNA interacts with and modulates p-ERM proteins in human somatic cells. Nat Commun, 6():7316(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Human, pull-down, LC-MS/MS |
Contact List
Li Mao |
contact affiliation | Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, Baltimore MD |
contact email | LMao@umaryland.edu |
lab head | |
David Clark |
contact affiliation | UMB School of Dentistry |
contact email | david.clark@umaryland.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002076
- Label: PRIDE project
- Name: A piRNA like small RNA interacts with and modulates p-ERM proteins in human somatic cells