PXD002075

PXD002075 is an original dataset announced via ProteomeXchange.

Title	ESCRTIII-associated protein AtALIX mediates high affinity phosphate transporter trafficking to maintain phosphate homeostasis in Arabidopsis
Description	Prior to the release of their cargoes into the vacuolar lumen, sorting endosomes mature into multivesicular bodies (MVB) through the action of ENDOSOMAL COMPLEX REQUIRED FOR TRANSPORT (ESCRT) protein complexes. MVB-mediated sorting of high affinity phosphate transporters (PHT1) to the vacuole limits their plasma membrane levels under phosphate sufficient conditions, a process that allows plants to maintain phosphate homeostasis. We describe here AtALIX, a cytosolic protein that associates to MVB by interacting with ESCRT-III subunit SNF7 and enables PHT1;1 trafficking to the vacuole. Thus, we show that the partial loss of function mutant Atalix-1 displays reduced vacuolar degradation of PHT1;1. AtALIX versions containing the Atalix-1 mutation showed reduced interaction with SNF7, providing a simple molecular explanation for impaired cargo trafficking in Atalix-1 mutants. Indeed, Atalix-1 mutation also hampered vacuolar sorting of brassinosteroid receptor BRI1. In line with a presumed broad target spectrum, Atalix-1 mutation is pleiotropic, provoking reduced plant growth, late flowering and altered vacuole morphogenesis, whereas null mutants are lethal, indicating that AtALIX controls diverse processes in plants being essential for their life.
HostingRepository	PRIDE
AnnounceDate	2015-09-09
AnnouncementXML	Submission_2015-09-09_06:12:05.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002075
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	J. Alberto Medina-Aunon
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-17 00:54:18	ID requested
⏵ 1	2015-09-09 06:12:06	announced

Cardona-L, ó, pez X, Cuyas L, Mar, í, n E, Rajulu C, Irigoyen ML, Gil E, Puga MI, Bligny R, Nussaume L, Geldner N, Paz-Ares J, Rubio V, ESCRT-III-Associated Protein ALIX Mediates High-Affinity Phosphate Transporter Trafficking to Maintain Phosphate Homeostasis in Arabidopsis. Plant Cell, 27(9):2560-81(2015) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, MS

Vicente Rubio Muñoz
contact affiliation	Department Y centro: Plant Molecular Genetics, Spanish National Center of Biotechnology (CNB-CSIC).
contact email	vrubio@cnb.csic.es
lab head
J. Alberto Medina-Aunon
contact affiliation	Proteomics - Bioinformatics
contact email	jamedina@proteored.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002075
     1. Label: PRIDE project
     2. Name: ESCRTIII-associated protein AtALIX mediates high affinity phosphate transporter trafficking to maintain phosphate homeostasis in Arabidopsis