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PXD002075

PXD002075 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleESCRTIII-associated protein AtALIX mediates high affinity phosphate transporter trafficking to maintain phosphate homeostasis in Arabidopsis
DescriptionPrior to the release of their cargoes into the vacuolar lumen, sorting endosomes mature into multivesicular bodies (MVB) through the action of ENDOSOMAL COMPLEX REQUIRED FOR TRANSPORT (ESCRT) protein complexes. MVB-mediated sorting of high affinity phosphate transporters (PHT1) to the vacuole limits their plasma membrane levels under phosphate sufficient conditions, a process that allows plants to maintain phosphate homeostasis. We describe here AtALIX, a cytosolic protein that associates to MVB by interacting with ESCRT-III subunit SNF7 and enables PHT1;1 trafficking to the vacuole. Thus, we show that the partial loss of function mutant Atalix-1 displays reduced vacuolar degradation of PHT1;1. AtALIX versions containing the Atalix-1 mutation showed reduced interaction with SNF7, providing a simple molecular explanation for impaired cargo trafficking in Atalix-1 mutants. Indeed, Atalix-1 mutation also hampered vacuolar sorting of brassinosteroid receptor BRI1. In line with a presumed broad target spectrum, Atalix-1 mutation is pleiotropic, provoking reduced plant growth, late flowering and altered vacuole morphogenesis, whereas null mutants are lethal, indicating that AtALIX controls diverse processes in plants being essential for their life.
HostingRepositoryPRIDE
AnnounceDate2015-09-09
AnnouncementXMLSubmission_2015-09-09_06:12:05.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD002075
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJ. Alberto Medina-Aunon
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListNo PTMs are included in the dataset
Instrument4800 Proteomics Analyzer
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-04-17 00:54:18ID requested
12015-09-09 06:12:06announced
Publication List
Cardona-L, ó, pez X, Cuyas L, Mar, í, n E, Rajulu C, Irigoyen ML, Gil E, Puga MI, Bligny R, Nussaume L, Geldner N, Paz-Ares J, Rubio V, ESCRT-III-Associated Protein ALIX Mediates High-Affinity Phosphate Transporter Trafficking to Maintain Phosphate Homeostasis in Arabidopsis. Plant Cell, 27(9):2560-81(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Arabidopsis, MS
Contact List
Vicente Rubio Muñoz
contact affiliationDepartment Y centro: Plant Molecular Genetics, Spanish National Center of Biotechnology (CNB-CSIC).
contact emailvrubio@cnb.csic.es
lab head
J. Alberto Medina-Aunon
contact affiliationProteomics - Bioinformatics
contact emailjamedina@proteored.org
dataset submitter
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Dataset FTP location
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PRIDE project URI
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