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PXD002071

PXD002071 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDistinct Serum Proteome Profiles Associated with Collagen-induced Arthritis, and Complete Freund’s Adjuvant-induced Inflammation in CD38-/- mice: the Discriminative Power of Protein Species or Proteoforms. Spot 269_Spot 500
DescriptionCollagen-type-II-induced arthritis (CIA) is an autoimmune disease, which involves a complex host systemic response including inflammatory and autoimmune reactions. CIA in C57BL/6 mice resembles human rheumatoid arthritis (RA) in terms of its disease course, histological findings, and in its response to commonly used anti-arthritic drugs. In this study, the goal has been to identify proteins from serum that change in their abundance in CD38-KO versus WT mice which may reflect their distinct response to an antigen-challenge that induces the development of an autoimmune disease.CIA is milder in CD38-/- than in Wild-type (WT) mice. We analyzed the sera from CD38-/- versus WT mice either with arthritis (CIA+), with no arthritis (CIA-), or with inflammation (Complete Freund’s adjuvant (CFA)-treated mice). To decrease the dynamic concentration range of serum a combinatorial ligand library composed of hexapeptides was used (called ProteoMiner). ProteoMiner-equalized serum samples were then subjected to 2D-DiGE and MS-MALDI-TOF/TOF analyses to identify proteins that changed in their relative abundances. Multivariate analyses revealed that a multi-protein signature (n = 28) was able to discriminate CIA+ from CIA- mice, and WT from CD38-/- mice within each condition. Likewise, a distinct multi-protein signature (n = 16) was identified which differentiated CIA+CD38-/- mice from CIA+ WT mice, and lastly, a third multi-protein signature (n = 18) indicated that CD38-/- and WT mice could be segregated in response to CFA treatment This approach allows the identification of multiple protein species, or proteoforms of a given protein in a single analysis, and therefore, to focus the interest in fully characterize just the protein species that differ in abundance.
HostingRepositoryPRIDE
AnnounceDate2015-07-17
AnnouncementXMLSubmission_2015-07-17_10:05:07.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD002071
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAntonio Rosal-Vela
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
Instrumentultraflex
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-04-15 02:04:39ID requested
12015-07-17 10:05:08announced
Publication List
Rosal-Vela A, Garc, í, a-Rodr, í, guez S, Postigo J, Iglesias M, Longobardo V, Lario A, Merino J, Merino R, Zubiaur M, Sancho J, mice: The discriminative power of protein species or proteoforms. Proteomics, 15(19):3382-93(2015) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: protein species, proteoforms, low-abundance proteins, arthritis, inflammation, CD38
Contact List
Jaime
contact affiliationInstituto de Parasitología y Biomedicina "López - Neyra", CSIC Departamento de Biología Celular e Inmunología Parque Tecnológico de Ciencias de la Salud, Avda. del Conocimiento, s/n 18016 Armilla (Granada). Spain
contact emailgranada@ipb.csic.es
lab head
Antonio Rosal-Vela
contact affiliationPostDoc researcher
contact emailarosal@ipb.csic.es
dataset submitter
Full Dataset Link List
Dataset FTP location
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