PXD002067

PXD002067 is an original dataset announced via ProteomeXchange.

Title	Wheat Allergens MALDI-TOF/TOF
Description	The aim of the work was to test relatively simple proteomics approach based on phenol extraction and two-dimensional gel electrophoresis (2-DE) with 7cm immobilized pH gradient strips for the determination of clinically relevant proteins in wheat grain. Using this approach, 157 2-DE spots were quantified in biological triplicate, out of which 55 were identified by MALDI-TOF/TOF mass spectrometry. Clinically relevant proteins associated with celiac disease, wheat dependent exercise induced anaphylaxis, baker´s asthma, and food allergy, were detected in 24 2-DE spots. However, alcohol-soluble gliadins were not detected with this approach. The comparison with recent quantitative study suggested that gel-based and gel-free proteomics approaches are complementary for the detection and quantification of clinically relevant proteins in wheat grain.
HostingRepository	PRIDE
AnnounceDate	2017-08-24
AnnouncementXML	Submission_2017-08-24_00:30:40.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002067
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maksym Danchenko
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-14 05:56:17	ID requested
1	2015-06-08 02:11:34	announced
2	2017-06-29 04:12:06	announced	Updated publication reference for PubMed record(s): 25365400.
3	2017-06-29 04:17:35	announced	Updated publication reference for PubMed record(s): 25365400.
4	2017-08-23 23:34:03	announced	Updated project metadata.
⏵ 5	2017-08-24 00:30:42	announced	Updated project metadata.

Fekecsov, á S, Danchenko M, Uvackova L, Skultety L, Hajduch M, Using 7 cm immobilized pH gradient strips to determine levels of clinically relevant proteins in wheat grain extracts. Front Plant Sci, 6():433(2015) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Triticum aestivum, gel-based quantification, MALDI-TOF/TOF, grain, allergen

Martin Hajduch
contact affiliation	Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Slovakia
contact email	hajduch@savba.sk
lab head
Maksym Danchenko
contact affiliation	Institute of Virology, Biomedical Research Center SAS
contact email	maxymdan@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002067
     1. Label: PRIDE project
     2. Name: Wheat Allergens MALDI-TOF/TOF