PXD002066

PXD002066 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics Illuminates A Functional Interaction Between Mouse Homolog Of Diaphanous 2 (mDia2) And The Proteasome
Description	Formin mDia2 is a cytoskeleton-regulatory protein that switches reversibly between a closed, auto-inhibited and an open, active conformation. Although the open conformation of mDia2 induces actin assembly thereby controlling many cellular processes, mDia2 possesses also actin-independent and conformation-insensitive scaffolding roles related to microtubules and p53, respectively. Thus, we hypothesise that mDia2 may have other unappreciated functions and regulatory modes. Here we identify and validate proteasome and Ubiquitin as genuine mDia2-interacting partners using quantitative proteomics and a multi-disciplinary approach, respectively.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:28:53.345.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Onno Bleijerveld
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	isotope labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-14 03:35:42	ID requested
1	2017-10-24 03:02:39	announced
⏵ 2	2024-10-22 04:28:56	announced	2024-10-22: Updated project metadata.

Isogai T, van der Kammen R, Bleijerveld OB, Goerdayal SS, Argenzio E, Altelaar AF, Innocenti M, Quantitative Proteomics Illuminates a Functional Interaction between mDia2 and the Proteasome. J Proteome Res, 15(12):4624-4637(2016) [pubmed]

curator keyword: Biological

submitter keyword: Human, HeLa, mDia2, proteasome, ubiquitin

A.F. Maarten Altelaar
contact affiliation	The Netherlands Cancer Institute Proteomics Facility
contact email	m.altelaar@uu.nl
lab head
Onno Bleijerveld
contact affiliation	The Netherlands Cancer Institute
contact email	o.bleijerveld@nki.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD002066

PRIDE project URI

• PRIDE
  1. PXD002066
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics Illuminates A Functional Interaction Between Mouse Homolog Of Diaphanous 2 (mDia2) And The Proteasome