PXD002063

PXD002063 is an original dataset announced via ProteomeXchange.

Title	MPP profiling in yeast
Description	The majority of mitochondrial preproteins is targeted via N-terminal presequences that are cleaved upon import into the organelle. The essential mitochondrial processing protease (MPP) is assumed to cleave the majority of incoming precursors. However, only few substrate proteins of MPP have been experimentally determined and analysis of a broad range of substrates is missing so far. Here, we present the first systematic approach to identify substrate proteins of MPP. We used a temperature-sensitive mutant of the MPP subunit Mas1 grown at non-permissive temperature. Analysis of highly purified mitochondria by quantitative N-terminal ChaFRADIC led to the identification of 85 potential MPP substrate proteins. Deduction of the cleaved presequences shows that arginine in position -2 is the main determinant of MPP recognition. Interestingly, several non-processed proteins were increased in mas1 mutant mitochondria revealing a novel mitochondrial proteotoxic stress response that ultimately leads to an increased membrane potential presumably to balance/compensate the impairment of the major presequence processing peptidase.
HostingRepository	PRIDE
AnnounceDate	2015-10-14
AnnouncementXML	Submission_2015-10-13_23:52:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Maria Burkhart
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-14 00:50:45	ID requested
⏵ 1	2015-10-13 23:52:13	announced

Burkhart JM, Taskin AA, Zahedi RP, V, ö, gtle FN, Quantitative Profiling for Substrates of the Mitochondrial Presequence Processing Protease Reveals a Set of Nonsubstrate Proteins Increased upon Proteotoxic Stress. J Proteome Res, 14(11):4550-63(2015) [pubmed]

curator keyword: Biological

submitter keyword: Mitochondria, presequence import pathway, mitochondrial processing protease MPP, ChaFRADIC, mitochondrial stress response

René Zahedi
contact affiliation	Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V.
contact email	zahedi@isas.de
lab head
Julia Maria Burkhart
contact affiliation	Department of bioanalytics
contact email	julia.burkhart@isas.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002063
     1. Label: PRIDE project
     2. Name: MPP profiling in yeast