PXD002040

PXD002040 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of the extracellular matrix (slime sheath) of Dictyostelium discoideum
Description	Dictyostelium discoideum has historically served as a model system for cell and developmental biology, but recently it has gained increasing attention as a model for the study of human diseases. The extracellular matrix (ECM) of this eukaryotic microbe serves multiple essential functions during development. It not only provides structural integrity to the moving multicellular pseudoplasmodium, or slug, it also provides components that regulate cell motlity and differentiation. An LC/MS/MS analysis of slug ECM revealed the presence of a large number of proteins in two wild-type strains, NC4 and WS380B. GO annotation identified proteins involved in binding, as well as proteins that modulate metabolic processes, cell movement, and multicellular development. In addition, this proteomic analysis identified numerous expected, as well as unexpected components.
HostingRepository	PRIDE
AnnounceDate	2015-07-29
AnnouncementXML	Submission_2015-07-29_00:31:15.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002040
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Robert Huber
SpeciesList	scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689;
ModificationList	Oxidation; Dehydrated; Ammonia-loss; Acetyl; Carbamidomethyl; Deamidated
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-10 05:54:53	ID requested
1	2015-07-21 22:31:11	announced
⏵ 2	2015-07-29 00:31:16	announced	Updated project metadata.

Huber RJ, O'Day DH, Proteomic profiling of the extracellular matrix (slime sheath) of Dictyostelium discoideum. Proteomics, 15(19):3315-9(2015) [pubmed]

curator keyword: Biological

submitter keyword: Development, Dictyostelium discoideum, Extracellular matrix, Model system, Proteomics

Robert Joseph Huber
contact affiliation	Massachusetts General Hospital Center for Human Genetic Research
contact email	rhuber@mgh.harvard.edu
lab head
Robert Huber
contact affiliation	Massachusetts General Hospital
contact email	rhuber@mgh.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/07/PXD002040

PRIDE project URI

• PRIDE
  1. PXD002040
     1. Label: PRIDE project
     2. Name: Proteomic profiling of the extracellular matrix (slime sheath) of Dictyostelium discoideum