PXD002032

PXD002032 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of HMGA1-silencing
Description	HMGA1 is a well-established oncogene and is a master regulator in breast cancer cells controlling the shift from a non-tumorigenic epithelial-like phenotype towards a highly aggressive mesenchymal-like one. In this work we compared HMGA1-silenced versus control MDA-MB-231 by means of a label free shotgun proteomics approach and crossed these data with DNA microarray expression profile obtained on the same cells. Resulting data were then filtered for genes linked to poor prognosis in breast cancer gene expression meta-datasets. This workflow allows us to establish a small molecular signature composed by 21 members with a prognostic value as regards overall-, recurrence free-, and distant metastasis free-survival in breast cancer.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:24:08.063.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-09 02:29:55	ID requested
1	2018-10-17 08:06:19	announced
⏵ 2	2024-10-22 04:24:10	announced	2024-10-22: Updated project metadata.

10.1074/mcp.M115.050401;

Maurizio E, Wi, ś, niewski JR, Ciani Y, Amato A, Arnoldo L, Penzo C, Pegoraro S, Giancotti V, Zambelli A, Piazza S, Manfioletti G, Sgarra R, Translating Proteomic Into Functional Data: An High Mobility Group A1 (HMGA1) Proteomic Signature Has Prognostic Value in Breast Cancer. Mol Cell Proteomics, 15(1):109-23(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: label free proteomics, high mobility protein HMGA1, filter aided sample preparation,Breast cancer

Jacek R Wisniewski
contact affiliation	Max-Planck Intitute of Biochemistry, Dept. Proteomics and Signal Transduction
contact email	jwisniew@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD002032

PRIDE project URI

• PRIDE
  1. PXD002032
     1. Label: PRIDE project
     2. Name: Proteomic analysis of HMGA1-silencing