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PXD002029

PXD002029 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative proteome stability analysis of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human colon mucosal biopsies
DescriptionClinical proteomics research is in part limited by the availability of clinical samples. However, large biobanks exist worldwide containing formalin-fixed and paraffin embedded samples and samples stored in RNAlater. The extraction of proteins for proteome analysis from samples prepared by either method has been demonstrated. However, the impact of the preservation method on the result of a quantitative proteome analysis remains largely uninvestigated. We, therefore, conducted a proteome analysis of human colon mucosal biopsies where the material had been preserved accordingly. Twenty-four colon mucosal biopsies were extracted from the sigmoideum by endoscopy from two participants. The biopsies were either directly-frozen, stabilized in RNAlater, or stabilized by formaldehyde fixation immediately or incubated for 30 min to simulate a clinical situation, followed by paraffin embedding. The impact of the preservation method on the result of a proteome analysis was characterized by high throughput gel free quantitative proteomics.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:24:27.741.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTue Bjerg Bennike
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-04-08 01:49:13ID requested
12015-11-23 14:12:20announced
22016-03-09 02:19:01announcedUpdated publication reference for PubMed record(s): 26937473.
32024-10-22 04:24:35announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.dib.2016.01.061;
Bennike TB, Kastaniegaard K, Padurariu S, Gaihede M, Birkelund S, Andersen V, Stensballe A, Proteome stability analysis of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human colon mucosal biopsies. Data Brief, 6():942-7(2016) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Human, snap-frozen, proteomics, mucosa, LC-MS, FFPE, RNAlater, colon, stability
Contact List
Allan Stensballe
contact affiliationLaboratory for Medical Mass Spectrometry Department of Health Science and Technology Aalborg University Denmark
contact emailas@hst.aau.dk
lab head
Tue Bjerg Bennike
contact affiliationAalborg University
contact emailtbe@hst.aau.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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