PXD002026

PXD002026 is an original dataset announced via ProteomeXchange.

Title	Proteome of microparticles derived from Mycobacterium tuberculosis infected macrophages
Description	Microparticles (MP) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M.tb) infection of macrophages increased the release of MPs, and these MPs induced a proinflammatory response from uninfected macrophages in vitro and in vivo following their transfer into uninfected mice. To determine how M.tb infection modulates the protein composition of the MPs, and if this contributes to their proinflammatory properties, we compared the proteomes of MPs derived from M.tb-infected (TBinf-MP) and uninfected human THP-1 monocytic cells. MP proteins were analysed by GeLC-MS/MS with spectral counting revealing 68 proteins with statistically significant differential abundances. The 42 proteins increased in abundance in TBinf-MPs included proteins associated with immune function (7), lysosomal/endosomal maturation (4), vesicular formation (12), nucleosome proteins (4) and antigen processing (9). Prominent among these were the type I interferon inducible proteins, ISG15, IFIT1, IFIT2, and IFIT3. Exposure of uninfected THP-1 cells to TBinf-MPs induced increased gene expression of isg15, ifit1, ifit2, and ifit3 and the release of proinflammatory cytokines. These proteins may regulate the proinflammatory potential of the MPs and provide candidate biomarkers for M.tb infection.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:52.085.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002026
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nathan Hare
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss; Propionamide; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Velos; QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-07 06:24:22	ID requested
1	2022-02-28 02:22:32	announced
⏵ 2	2023-11-14 08:48:52	announced	2023-11-14: Updated project metadata.

10.6019/PXD002026;

curator keyword: Biomedical

submitter keyword: macrophage, GeLC/MS,Microparticle, mycobacterium tuberculosis

Dr Bernadette Saunders
contact affiliation	Tuberculosis Research Program, Centenary Institute, Sydney, NSW, Australia; School of Medical Sciences, The University of Sydney, NSW, Australia
contact email	b.saunders@centenary.org.au
lab head
Nathan Hare
contact affiliation	Centenary Institute of Cancer Medicine and Cell Biology
contact email	n.hare@centenary.org.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD002026

PRIDE project URI

• PRIDE
  1. PXD002026
     1. Label: PRIDE project
     2. Name: Proteome of microparticles derived from Mycobacterium tuberculosis infected macrophages