PXD002025

PXD002025 is an original dataset announced via ProteomeXchange.

Title	Liver lipid metabolism is altered by increased circulating estrogen to androgen ratio in the liver of male mouse
Description	Estrogens are suggested to lower the risk of developing metabolic syndrome in both sexes. In this study, we investigated how the increased circulating estrogen-to-androgen ratio (E/A) alters liver lipid metabolism in males. Mice expressing human aromatase enzyme (AROM+ mice), and thus having high circulating E/A, were used as a model. Proteomics and gene expression analyses indicated an increase in the peroxisomal ß-oxidation in the liver of AROM+ mice as compared with their wild type littermates. Correspondingly, metabolomic analysis revealed a decrease in the amount of phosphatidylcholines with long-chain fatty acids in the plasma. With interest we note that the expression of Cyp4a12a enzyme, which specifically metabolizes arachidonic acid (AA) to 20-hydroxy AA, was dramatically decreased in the AROM+ liver. As a consequence, increased amounts of phospholipids having AA as a fatty acid tail were detected in the plasma of the AROM+ mice. Overall, these observations demonstrate that high circulating E/A in males is linked to indicators of higher peroxisomal ß -oxidation and lower AA metabolism in the liver. Furthermore, the plasma phospholipid profile reflects the changes in the liver lipid metabolism.
HostingRepository	PRIDE
AnnounceDate	2015-12-23
AnnouncementXML	Submission_2016-01-06_00:33:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anni Vehmas
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-07 06:21:13	ID requested
1	2015-09-03 03:11:28	announced
2	2016-01-06 00:32:48	announced	Updated publication reference for PubMed record(s): 26321463, 26691839.
⏵ 3	2016-01-06 00:33:58	announced	Updated publication reference for PubMed record(s): 26321463, 26691839.

Pursiheimo A, Vehmas AP, Afzal S, Suomi T, Chand T, Strauss L, Poutanen M, Rokka A, Corthals GL, Elo LL, Optimization of Statistical Methods Impact on Quantitative Proteomics Data. J Proteome Res, 14(10):4118-26(2015) [pubmed]

Vehmas AP, Adam M, Laajala TD, Kastenm, ü, ller G, Prehn C, Rozman J, Ohlsson C, Fuchs H, Hrab, ě, de Angelis M, Gailus-Durner V, Elo LL, Aittokallio T, Adamski J, Corthals G, Poutanen M, Strauss L, Liver lipid metabolism is altered by increased circulating estrogen to androgen ratio in male mouse. J Proteomics, 133():66-75(2016) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Liver, aromatase, male mouse, label free quantitative proteomics, metabolomics, phospholipid

Garry L. Corthals
contact affiliation	Current: Van 't Hoff Institute for Molecular Sciences, University of Amsterdam, The Netherlands; Previous: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland
contact email	G.L.Corthals@uva.nl
lab head
Anni Vehmas
contact affiliation	Turku Centre for Biotechnology, University of Turku and Åbo Akademi University
contact email	avehmas@btk.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/12/PXD002025

PRIDE project URI

• PRIDE
  1. PXD002025
     1. Label: PRIDE project
     2. Name: Liver lipid metabolism is altered by increased circulating estrogen to androgen ratio in the liver of male mouse