PXD002025 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Liver lipid metabolism is altered by increased circulating estrogen to androgen ratio in the liver of male mouse |
Description | Estrogens are suggested to lower the risk of developing metabolic syndrome in both sexes. In this study, we investigated how the increased circulating estrogen-to-androgen ratio (E/A) alters liver lipid metabolism in males. Mice expressing human aromatase enzyme (AROM+ mice), and thus having high circulating E/A, were used as a model. Proteomics and gene expression analyses indicated an increase in the peroxisomal ß-oxidation in the liver of AROM+ mice as compared with their wild type littermates. Correspondingly, metabolomic analysis revealed a decrease in the amount of phosphatidylcholines with long-chain fatty acids in the plasma. With interest we note that the expression of Cyp4a12a enzyme, which specifically metabolizes arachidonic acid (AA) to 20-hydroxy AA, was dramatically decreased in the AROM+ liver. As a consequence, increased amounts of phospholipids having AA as a fatty acid tail were detected in the plasma of the AROM+ mice. Overall, these observations demonstrate that high circulating E/A in males is linked to indicators of higher peroxisomal ß -oxidation and lower AA metabolism in the liver. Furthermore, the plasma phospholipid profile reflects the changes in the liver lipid metabolism. |
HostingRepository | PRIDE |
AnnounceDate | 2015-12-23 |
AnnouncementXML | Submission_2016-01-06_00:33:56.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Anni Vehmas |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-04-07 06:21:13 | ID requested | |
1 | 2015-09-03 03:11:28 | announced | |
2 | 2016-01-06 00:32:48 | announced | Updated publication reference for PubMed record(s): 26321463, 26691839. |
⏵ 3 | 2016-01-06 00:33:58 | announced | Updated publication reference for PubMed record(s): 26321463, 26691839. |
Publication List
Pursiheimo A, Vehmas AP, Afzal S, Suomi T, Chand T, Strauss L, Poutanen M, Rokka A, Corthals GL, Elo LL, Optimization of Statistical Methods Impact on Quantitative Proteomics Data. J Proteome Res, 14(10):4118-26(2015) [pubmed] |
Vehmas AP, Adam M, Laajala TD, Kastenm, ü, ller G, Prehn C, Rozman J, Ohlsson C, Fuchs H, Hrab, ě, de Angelis M, Gailus-Durner V, Elo LL, Aittokallio T, Adamski J, Corthals G, Poutanen M, Strauss L, Liver lipid metabolism is altered by increased circulating estrogen to androgen ratio in male mouse. J Proteomics, 133():66-75(2016) [pubmed] |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: Liver, aromatase, male mouse, label free quantitative proteomics, metabolomics, phospholipid |
Contact List
Garry L. Corthals |
contact affiliation | Current: Van 't Hoff Institute for Molecular Sciences, University of Amsterdam, The Netherlands; Previous: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland |
contact email | G.L.Corthals@uva.nl |
lab head | |
Anni Vehmas |
contact affiliation | Turku Centre for Biotechnology, University of Turku and Åbo Akademi University |
contact email | avehmas@btk.fi |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002025
- Label: PRIDE project
- Name: Liver lipid metabolism is altered by increased circulating estrogen to androgen ratio in the liver of male mouse