PXD002024

PXD002024 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis of methanoarchaeon Methanohalophilus portucalensis FDF1T reveals diverse functions in methanogenesis and osmoadaptation
Description	Methane is a well-known factor in green house effect and also could be an alternative energy, which produced via methanogenesis by methanogenic archaea. Methanohalophilus portucalensis FDF1T is a halophilic methylotrophic methanogen and has been used as a model to study osmoadaptation in fluctuated abiotic environmental conditions. Here we report a genome-wide phosphoproteomic analysis of M. portucalensis FDF1T to uncover the possible phosphorylation-mediated regulations in methanogenesis and osmoadaptation. According to the biological function analysis revealed 20.6% of total 149 phosphoproteins were involved in the regulation of the single carbon metabolism for methanogenesis and osmoadaptation. Together with genome sequencing and phosphoproteomic analysis, we provided the first evidence to indicate protein phosphorylation networks in regulating the pyrrolysine (Pyl) mediated translation of methylotrophic methyltransferase. Furthermore, the functional assays of glycine sarcosine N-methyltransferase revealed that the enzymatic activity was modulated by threonine phosphorylation. Collectively, our results not only provide new insight into phosphorylation-mediated regulations in single carbon energy metabolism but also clearly demonstrate that phosphorylation plays a critical role in osmolyte betaine synthesis through a phospho-switch mechanism.
HostingRepository	PRIDE
AnnounceDate	2016-07-05
AnnouncementXML	Submission_2016-07-05_03:53:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ShuJung Lai
SpeciesList	scientific name: Methanohalophilus; NCBI TaxID: 2175;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-07 06:19:28	ID requested
⏵ 1	2016-07-05 03:53:34	announced

Wu WL, Lai SJ, Yang JT, Chern J, Liang SY, Chou CC, Kuo CH, Lai MC, Wu SH, Phosphoproteomic analysis of Methanohalophilus portucalensis FDF1(T) identified the role of protein phosphorylation in methanogenesis and osmoregulation. Sci Rep, 6():29013(2016) [pubmed]

curator keyword: Biological

submitter keyword: methanogenesis, phosphorylation modificatioin, Methanohalophilus portucalensis

Shu-Jung Lai
contact affiliation	Institute of Biological Chemistry, Taipei, Taiwan
contact email	sjlai01@gmail.com
lab head
ShuJung Lai
contact affiliation	postdoc
contact email	sjlai01@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002024
     1. Label: PRIDE project
     2. Name: Phosphoproteomic analysis of methanoarchaeon Methanohalophilus portucalensis FDF1T reveals diverse functions in methanogenesis and osmoadaptation