PXD002001

PXD002001 is an original dataset announced via ProteomeXchange.

Title	Human HeLa SILAC LC MS/MS - Hypoxia Strongly Affects Mitochondrial Ribosomal Proteins and Translocases, as Shown by Quantitative Proteomics of HeLa Cells
Description	Human HeLa cells were either kept in a hypoxic environment or under normoxic conditions. 137 proteins were found to be regulated, with maximum alteration of 18-fold. In particular, three clusters of differentially regulated proteins were identified, showing significant up-regulation of glycolysis and down-regulation of mitochondrial ribosomal proteins and translocases. This interaction is likely orchestrated by HIF-1. We also investigated the effect of hypoxia on the cell cycle, which is arrested at a restriction point in G1, and shows a prolonged S phase under these conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:18:40.971.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Koehler
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	(R)-5-oxo-1; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-31 06:06:37	ID requested
1	2017-01-02 05:18:35	announced
2	2017-10-24 04:12:50	announced	Updated project metadata.
⏵ 3	2024-10-22 04:18:42	announced	2024-10-22: Updated project metadata.

10.1155/2015/678527;

Bousquet PA, Sandvik JA, Arntzen M Ø, Jeppesen Edin NF, Christoffersen S, Krengel U, Pettersen EO, Thiede B, Hypoxia Strongly Affects Mitochondrial Ribosomal Proteins and Translocases, as Shown by Quantitative Proteomics of HeLa Cells. Int J Proteomics, 2015():678527(2015) [pubmed]

curator keyword: Biological

submitter keyword: Human, hypoxia, HeLa SILAC, LC-MS/MS

Bernd Thiede
contact affiliation	University of Oslo Department of Biosciences Cancer Proteomics P.O. Box 1066 Blindern, 0316 Oslo, Norway
contact email	bernd.thiede@biotek.uio.no
lab head
Christian Koehler
contact affiliation	UiO
contact email	christian.koehler@biotek.uio.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD002001

PRIDE project URI

• PRIDE
  1. PXD002001
     1. Label: PRIDE project
     2. Name: Human HeLa SILAC LC MS/MS - Hypoxia Strongly Affects Mitochondrial Ribosomal Proteins and Translocases, as Shown by Quantitative Proteomics of HeLa Cells