PXD001992

PXD001992 is an original dataset announced via ProteomeXchange.

Title	Hepatitis C-like viruses are produced in cells from rabbit and hare DNA
Description	Hepatitis C virus (HCV), a major causative agent of acute and chronic liver disease, belongs to the Flaviviridæ family and contains a single-strand positive-sense RNA genome, which upon virus entry and uncoating, functions as mRNAs and thus can be directly translated into proteins by host cell machinery. To date the HCV origin remains unclear and HCV life cycle and pathogenesis are not enlightened processes due to the absence of HCV efficient cell cultures systems or animals models. Here we show that rabbit and hare HCV-like viruses, RHCV and HHCV respectively, are formed after the inoculation of genomic DNA in Madin-Darby bovine kidney cell line cultures. RHCV is closely related to the HCV-1a/HCV-1b genotypes and HHCV is more closely related to the HCV-1b genotype. These findings could contribute to the understanding of HCV origin as well as clarify the virus life cycle, pathogenesis, evolution and diversity.
HostingRepository	PRIDE
AnnounceDate	2016-01-21
AnnouncementXML	Submission_2016-01-21_03:35:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Hepatitis C virus; NCBI TaxID: 11103;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-30 01:23:05	ID requested
⏵ 1	2016-01-21 03:35:19	announced

Silva E, Os, ó, rio H, Thompson G, Hepatitis C-like viruses are produced in cells from rabbit and hare DNA. Sci Rep, 5():14535(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: HCV, MALDI-TOF/TOF, RHCV, HHCV, Rabbit, Hare, DNA

Hugo Osorio
contact affiliation	Ipatimup / i3S, Porto, Portugal
contact email	hosorio@ipatimup.pt
lab head
Hugo Osorio
contact affiliation	IPATIMUP
contact email	hosorio@ipatimup.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001992
     1. Label: PRIDE project
     2. Name: Hepatitis C-like viruses are produced in cells from rabbit and hare DNA