PXD001989

PXD001989 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Drug-resistant Mycobacteria
Description	Tuberculosis, caused by the pathogen Mycobacterium tuberculosis is a worldwide public health threat. Mycobacterium tuberculosis is capable of resisting of various stresses in host cells including high levels of ROS and copper ions. To better understand the resistance mechanisms of mycobacteria to copper, we generated a copper-resistant strain of Mycobacterium smegmatis, mc2155-Cu from the selection of copper sulfate treated-bacteria. The mc2155-Cu strain has the 5-fold higher resistance to copper sulfate and the 2-fold higher resistance to isoniazid (INH) than its parental strain mc2155, respectively. Quantitative proteomics was carried out to find differentially expressed proteins between mc2155 and mc2155-Cu. Among 345 differentially expressed proteins, copper-translocating P-type ATPase was up-regulated, while all other ABC transporters were down-regulated in mc2155-Cu, suggesting copper-translocating P-type ATPase plays a crucial role in copper resistance. Results also indicated that the down-regulation of metabolic enzymes and decreases in cellular NAD, FAD, mycothiol, and glutamine levels in mc2155-Cu were responsible to its slow growth rate as compared to mc2155. Down-regulation of KatG2 expression in both protein and mRNA levels indicates the co-evolution of copper and INH resistance in copper resistance bacteria, and provides new evidences to understanding of the molecular mechanisms of survival of mycobacteria under stress conditions.
HostingRepository	PRIDE
AnnounceDate	2016-04-13
AnnouncementXML	Submission_2016-04-13_02:35:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuling Chen
SpeciesList	scientific name: Corynebacterineae; NCBI TaxID: 85007;
ModificationList	monohydroxylated residue; iodoacetamide derivatized amino-terminal residue; deaminated residue; TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-27 07:36:28	ID requested
⏵ 1	2016-04-13 02:35:07	announced

Chen Y, Yang F, Sun Z, Wang Q, Mi K, Deng H, Proteomic Analysis of Drug-Resistant Mycobacteria: Co-Evolution of Copper and INH Resistance. PLoS One, 10(6):e0127788(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: copper resistant, drug resistant, mycobacteria

Yuling Chen
contact affiliation	School of Life Science, Tsinghua University, China
contact email	chenyuling2010@yeah.net
lab head
Yuling Chen
contact affiliation	Tsinghua University
contact email	chenyuling2010@yeah.net
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD001989

PRIDE project URI

• PRIDE
  1. PXD001989
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Drug-resistant Mycobacteria