PXD001988 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | cell surface glycoproteome liver and lung |
Description | Purpose Cell surface proteins are the primary means for a cell to sense and interact with its environment and their dysregulation has been linked to numerous diseases. In particular, the identification of proteins specific to a single tissue type or to a given disease phenotype may enable the characterization of novel therapeutic targets. We tested here the feasibility of a cell surface proteomics approach to identify pertinent markers directly in a clinically relevant tissue. Experimental design We analyzed the cell surface proteome of freshly isolated primary heptatocytes using a glycocapture-specific approach combined with a robust bioinformatics filtering. Results Using primary lung epithelial cell cultures as negative controls, we identified 32 hepatocyte-specific cell surface proteins candidates. We used mRNA expression to select six markers that may provide adequate specificity for targeting therapeutics to the liver. Conclusions and clinical relevance We demonstrate the feasibility and the importance of conducting such studies directly in a clinically-relevant tissue. In particular, the cell surface proteome of freshly isolated hepatocytes differed substantially from cultured cell lines. |
HostingRepository | PRIDE |
AnnounceDate | 2015-07-22 |
AnnouncementXML | Submission_2015-07-22_05:21:15.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Axel Ducret |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-03-27 07:07:02 | ID requested | |
⏵ 1 | 2015-07-22 05:21:17 | announced | |
Publication List
Ducret A, Kux van Geijtenbeek S, R, รถ, der D, Simon S, Chin D, Berrera M, Gruenbaum L, Ji C, Cutler P, Identification of six cell surface proteins for specific liver targeting. Proteomics Clin Appl, 9(7-8):651-61(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: human, primary hepatocytes, epithelial lung cells, glycocapture, LC-MS, LTQ-Orbitrap |
Contact List
Axel Ducret |
contact affiliation | Roche Pharma Research and Early Development Roche Innovation Center Basel F. Hoffmann-La Roche Ltd CH-4070 Basel, Switzerland |
contact email | axel.ducret@roche.com |
lab head | |
Axel Ducret |
contact affiliation | F. Hoffmann-La Roche Ltd |
contact email | axel.ducret@roche.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001988
- Label: PRIDE project
- Name: cell surface glycoproteome liver and lung