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PXD001988

PXD001988 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titlecell surface glycoproteome liver and lung
DescriptionPurpose Cell surface proteins are the primary means for a cell to sense and interact with its environment and their dysregulation has been linked to numerous diseases. In particular, the identification of proteins specific to a single tissue type or to a given disease phenotype may enable the characterization of novel therapeutic targets. We tested here the feasibility of a cell surface proteomics approach to identify pertinent markers directly in a clinically relevant tissue. Experimental design We analyzed the cell surface proteome of freshly isolated primary heptatocytes using a glycocapture-specific approach combined with a robust bioinformatics filtering. Results Using primary lung epithelial cell cultures as negative controls, we identified 32 hepatocyte-specific cell surface proteins candidates. We used mRNA expression to select six markers that may provide adequate specificity for targeting therapeutics to the liver. Conclusions and clinical relevance We demonstrate the feasibility and the importance of conducting such studies directly in a clinically-relevant tissue. In particular, the cell surface proteome of freshly isolated hepatocytes differed substantially from cultured cell lines.
HostingRepositoryPRIDE
AnnounceDate2015-07-22
AnnouncementXMLSubmission_2015-07-22_05:21:15.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAxel Ducret
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListdeamidated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-03-27 07:07:02ID requested
12015-07-22 05:21:17announced
Publication List
Ducret A, Kux van Geijtenbeek S, R, รถ, der D, Simon S, Chin D, Berrera M, Gruenbaum L, Ji C, Cutler P, Identification of six cell surface proteins for specific liver targeting. Proteomics Clin Appl, 9(7-8):651-61(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: human, primary hepatocytes, epithelial lung cells, glycocapture, LC-MS, LTQ-Orbitrap
Contact List
Axel Ducret
contact affiliationRoche Pharma Research and Early Development Roche Innovation Center Basel F. Hoffmann-La Roche Ltd CH-4070 Basel, Switzerland
contact emailaxel.ducret@roche.com
lab head
Axel Ducret
contact affiliationF. Hoffmann-La Roche Ltd
contact emailaxel.ducret@roche.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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