PXD001985

PXD001985 is an original dataset announced via ProteomeXchange.

Title	CF sputum LC-MSMS
Description	Lung disease is the main cause of morbidity and mortality in cystic fibrosis (CF), and involves chronic infection by a destructive microbiota and perturbed innate and adaptive immune responses. Tissue damage is considered to be mediated mostly by proteases, but other bacterial and host factors may also play a role. To determine the presence of potentially injurious proteins we employed semi-quantitative Multidimensional Protein Identification Technology to identify sputum cellular proteins with consistently altered expression in CF compared to healthy controls. Ingenuity Pathway Analysis, Gene Ontology functions, protein abundance and correlation with lung function were used to infer their clinical significance. The CF proteome exhibited differential expression of proteins relating to Rho family small GTPase activity, immune cell movement and activation, generation of reactive oxygen species and dysregulation of cell death and proliferation. Compositional breakdown established neutrophil extracellular trap proteins as the consistently most abundant cellular proteins detected, while a further 13 biologically relevant proteins were found to correlate negatively with lung function. These findings expand the current understanding of the mechanisms underlying CF lung disease and identify sputum cell proteins which might be useful as markers of disease status, prognostic indicators, stratification determinants for treatment prescription or as therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2017-05-19
AnnouncementXML	Submission_2017-09-13_00:07:50.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001985
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	S Pattison
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Deamidated; Oxidation; iTRAQ8plex
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-27 01:21:27	ID requested
1	2017-05-19 02:57:52	announced
⏵ 2	2017-09-13 00:07:51	announced	Updated publication reference for PubMed record(s): 28679606.

Pattison SH, Gibson DS, Johnston E, Peacock S, Rivera K, Tunney MM, Pappin DJ, Elborn JS, . Eur Respir J, 50(1):(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: cystic fibrosis, sputum, LC-MSMS

Joseph Stuart Elborn
contact affiliation	Centre for Infection and Immunity, Queen’s University Belfast, Belfast, Northern Ireland, BT9 7BL, UK.
contact email	s.elborn@qub.ac.uk
lab head
S Pattison
contact affiliation	Centre for Infection and Immunity
contact email	s.pattison@qub.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD001985

PRIDE project URI

• PRIDE
  1. PXD001985
     1. Label: PRIDE project
     2. Name: CF sputum LC-MSMS