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PXD001981

PXD001981 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSecretome of P2X7 receptor
DescriptionIn order to determine P2X7R secretome we analyzed the proteins present in cell-free supernatants from wild-type (P2rx7+/+) or P2rx7-/- bone marrow-derived macrophages (BMDMs) polarized either to M1 or M2 and subsequently treated with ATP. BMDMs were primed with LPS (M1) or IL-4 (M2) for 4 hours and the proteins secreted during this step were extensively washed with PBS before ATP was added in fresh buffer. The complex mixture of proteins obtained in the macrophages supernatants after ATP stimulation were fractionated using one dimension gel electrophoresis and 10 bands were selected for LC-MS/MS analysis based in their presence in higher intensity in P2rx7+/+ supernatant compared with P2rx7-/- supernatant.
HostingRepositoryPRIDE
AnnounceDate2016-03-11
AnnouncementXMLSubmission_2016-03-11_10:20:13.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCarlos de Torre Minguela
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentThermo Finnigan instrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-03-26 06:50:45ID requested
12016-03-11 10:20:15announced
Publication List
de Torre-Minguela C, Barber, à, -Cremades M, G, ó, mez AI, Mart, í, n-S, á, nchez F, Pelegr, í, n P, Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process. Sci Rep, 6():22586(2016) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: Mouse, BMDM, P2X7R, LC-MS/MS
Contact List
Carlos de Torre Minguela
contact affiliationInflammation and Experimental Surgery Unit LAIB Building - 4th floor (lab 4.20) IMIB-Arrixaca Ctra. Buenavista s/n 30120 El Palmar (Murcia). Spain
contact emailcarlos.de3@um.es
lab head
Carlos de Torre Minguela
contact affiliationIMIB-Arrixaca
contact emaildetorre.minguela@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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