PXD001981 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Secretome of P2X7 receptor |
Description | In order to determine P2X7R secretome we analyzed the proteins present in cell-free supernatants from wild-type (P2rx7+/+) or P2rx7-/- bone marrow-derived macrophages (BMDMs) polarized either to M1 or M2 and subsequently treated with ATP. BMDMs were primed with LPS (M1) or IL-4 (M2) for 4 hours and the proteins secreted during this step were extensively washed with PBS before ATP was added in fresh buffer. The complex mixture of proteins obtained in the macrophages supernatants after ATP stimulation were fractionated using one dimension gel electrophoresis and 10 bands were selected for LC-MS/MS analysis based in their presence in higher intensity in P2rx7+/+ supernatant compared with P2rx7-/- supernatant. |
HostingRepository | PRIDE |
AnnounceDate | 2016-03-11 |
AnnouncementXML | Submission_2016-03-11_10:20:13.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Carlos de Torre Minguela |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Thermo Finnigan instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-03-26 06:50:45 | ID requested | |
⏵ 1 | 2016-03-11 10:20:15 | announced | |
Publication List
de Torre-Minguela C, Barber, à, -Cremades M, G, ó, mez AI, Mart, í, n-S, á, nchez F, Pelegr, í, n P, Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process. Sci Rep, 6():22586(2016) [pubmed] |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: Mouse, BMDM, P2X7R, LC-MS/MS |
Contact List
Carlos de Torre Minguela |
contact affiliation | Inflammation and Experimental Surgery Unit LAIB Building - 4th floor (lab 4.20) IMIB-Arrixaca Ctra. Buenavista s/n 30120 El Palmar (Murcia). Spain |
contact email | carlos.de3@um.es |
lab head | |
Carlos de Torre Minguela |
contact affiliation | IMIB-Arrixaca |
contact email | detorre.minguela@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/03/PXD001981 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001981
- Label: PRIDE project
- Name: Secretome of P2X7 receptor