PXD001980

PXD001980 is an original dataset announced via ProteomeXchange.

Title	Two distinct modes for propagation of histone PTMs across the cell cycle
Description	Epigenetic states defined by chromatin can be maintained through mitotic cell division. However, it remains unknown how histone-based information is transmitted. Here we combine nascent chromatin capture (NCC) and triple-SILAC labelling to track histone modifications and histone variants during DNA replication and across the cell cycle. We show that post-translational modifications (PTMs) are transmitted with parental histones to newly replicated DNA. Di- and tri-methylation marks are diluted two-fold upon DNA replication, as a consequence of new histone deposition. Importantly, within one cell cycle all PTMs are restored. In general, new histones are modified to mirror the parental histones. However, H3K9me3 and H3K27me3 are propagated by continuous modification of parental and new histones, because the establishment of these marks extends over several cell generations. Together, our results reveal how histone marks propagate and demonstrate that chromatin states oscillate within the cell cycle.
HostingRepository	PRIDE
AnnounceDate	2015-03-30
AnnouncementXML	Submission_2015-03-30_07:15:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; phosphorylated residue; monomethylated residue; dimethylated residue; N6-propanoyl-L-lysine
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-26 03:16:31	ID requested
⏵ 1	2015-03-30 07:15:31	announced

Alabert C, Barth TK, Rever, ó, n-G, ó, mez N, Sidoli S, Schmidt A, Jensen ON, Imhof A, Groth A, Two distinct modes for propagation of histone PTMs across the cell cycle. Genes Dev, 29(6):585-90(2015) [pubmed]

curator keyword: Biological

submitter keyword: NCC

Histone modifications

inheritance

chromatin

epigenetics

Axel Imhof
contact affiliation	Zentrallabor fuer Proteinanalytik BMC - LMU Munich Schillerstrasse 44 Munich D-80336 Germany
contact email	imhof@lmu.de
lab head
Andreas Schmidt
contact affiliation	Adolf-Butenandt-Institut
contact email	A.Schmidt@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/03/PXD001980

PRIDE project URI

• PRIDE
  1. PXD001980
     1. Label: PRIDE project
     2. Name: Two distinct modes for propagation of histone PTMs across the cell cycle