PXD001968

PXD001968 is an original dataset announced via ProteomeXchange.

Title	Bacterial persistence
Description	Bacterial persistence, found in dormant and starved cells, is a health threat due to transient antibiotic tolerance. Harnessing a novel method for persister generation, we determined the proteome, metabolite levels and the physiology of E.coli persisters in and during entry into dormancy and starvation. In contrast to starved persisters, dormant persisters present in nutrient-rich conditions produced energy and grew, while both types had extremely low metabolite pools. The proteome of dormant cells governed by starvation response reached a unique state characterized by diminished anabolism, stress response and preservation of central metabolism protein levels. While starved cells approaches the same proteome, the limited carbon and energy source did not allow them to reach it, which caused their higher sensitivity to certain antibiotics. We present a conceptual model in which depleted metabolite pools resulting from initial persistence triggers provide a primitive, feed-forward starvation signal that sustains the growing persistent phenotype.
HostingRepository	PRIDE
AnnounceDate	2016-09-27
AnnouncementXML	Submission_2016-09-26_18:02:36.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001968
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-24 09:36:01	ID requested
⏵ 1	2016-09-26 18:02:37	announced

Radzikowski JL, Vedelaar S, Siegel D, Ortega Á D, Schmidt A, Heinemann M, S stress response to metabolic flux limitation. Mol Syst Biol, 12(9):882(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: E. coli, antibiotic resistance

Alexander Schmidt
contact affiliation	Biozentrum, University of Basel, 4056 Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD001968

PRIDE project URI

• PRIDE
  1. PXD001968
     1. Label: PRIDE project
     2. Name: Bacterial persistence