This submission includes the mass spectrometry data for the manuscript by Mui et al. (E4orf4 interactome). This submission contains 16 files for E4orf4 WT or mutant interactions from HEK293 cells, acquired on LTQ Orbitrap Velos and LTQ Orbitrap Elite. See the README file within "Methods and Protocols" and the accompanying File description and peptide and interaction identification results.