PXD001951

PXD001951 is an original dataset announced via ProteomeXchange.

Title	The haemolymph proteome of Mytilus edulis: insights into the circulatory system and innate immunity of marine mussels
Description	Mussels are a group of sessile and filter-feeding invertebrates, and are key structural elements of many marine and freshwater ecosystems. The mussel haemolymph is the first defense line to pathogen infection and inflammation with an innate immune response. Despite the importance of this body fluid in determining the physiological condition of the animal, little is known about the molecular mechanisms underlying the cellular and humoral responses. Proteomic characterization of this body fluid has been hampered by the scarce Mytilus sp genomic data available. To systematic characterize the marine mussel Mytilus edulis hemolymph proteome, we have applied a mass spectrometry (nanoLC-MS/MS) strategy integrating genomics and transcriptomics data for proteins identification. After sample analysis and first identification based on MS/MS data comparison, proteins with unknown functions were annotated with blast using public database (nrNCBI) information. Overall 654 haemolymph proteins were identified with high confidence. The gene ontology analysis revealed that the majority of haemolymph proteins participate in primary cellular metabolic processes: energy production and metabolism of biomolecules. Nevertheless it also revealed a protein complement whose functions could be related to oxidative stress defense, xenobiotic detoxification, drug metabolism and immune response.
HostingRepository	PRIDE
AnnounceDate	2015-09-14
AnnouncementXML	Submission_2015-09-14_08:48:07.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001951
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexandre Campos
SpeciesList	scientific name: Mytilus edulis (Blue mussel); NCBI TaxID: 6550;
ModificationList	Oxidation; Dehydrated; Ammonia-loss; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-18 06:44:27	ID requested
⏵ 1	2015-09-14 08:48:09	announced

Campos A, Apraiz I, da Fonseca RR, Cristobal S, Shotgun analysis of the marine mussel Mytilus edulis hemolymph proteome and mapping the innate immunity elements. Proteomics, 15(23-24):4021-9(2015) [pubmed]

curator keyword: Biological

submitter keyword: Mytilus edulis, haemolymph, shotgun proteomics, biomarker, innate immunity

Susana Cristobal
contact affiliation	Department of Clinical and Experimental Medicine, Cell biology, Faculty of Health Science Linköping University, SE-581 83 Linköping, Sweden
contact email	susana.cristobal@liu.se
lab head
Alexandre Campos
contact affiliation	Linkoping University
contact email	alexandre.campos@liu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001951
     1. Label: PRIDE project
     2. Name: The haemolymph proteome of Mytilus edulis: insights into the circulatory system and innate immunity of marine mussels